| Literature DB >> 12837591 |
Yuzuru Shiio1, Robert N Eisenman, Eugene C Yi, Sam Donohoe, David R Goodlett, Ruedi Aebersold.
Abstract
A method to identify and quantify chromatin-associated proteins has been developed and applied to the analysis of changes in chromatin-associated proteins induced by Myc oncoprotein expression in human B lymphocytes. Chromatin-enriched fractions were isolated by differential detergent/salt extraction and analyzed by ICAT reagent labeling, multi-dimensional chromatography and tandem mass spectrometry. Many known chromatin-associated regulatory factors were identified and quantified. The method will be widely applicable to various biological systems and reveal changes in chromatin-associated regulatory factors that underlie biological phenomena.Entities:
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Year: 2003 PMID: 12837591 DOI: 10.1016/S1044-0305(03)00204-6
Source DB: PubMed Journal: J Am Soc Mass Spectrom ISSN: 1044-0305 Impact factor: 3.109