Literature DB >> 12836320

A new method for construction of Ti plasmid-less strains in Agrobacterium tumefaciens.

M Uraji1, K Suzuki, K Yoshida.   

Abstract

Agrobacterium tumefaciens harboring a Ti plasmid causes plant tumorigenesis and it's DNA transfer system have been extensively used in plant biotechnology. Because Ti plasmids are high stable in agrobacterial hosts, their curing is very difficult by conventional methods. Here, we introduced a novel curing method by using Ti plasmids incompatibility.

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Year:  2001        PMID: 12836320     DOI: 10.1093/nass/1.1.173

Source DB:  PubMed          Journal:  Nucleic Acids Res Suppl


  1 in total

1.  Plasmid conjugation from proteobacteria as evidence for the origin of xenologous genes in cyanobacteria.

Authors:  David Encinas; M Pilar Garcillán-Barcia; María Santos-Merino; Luis Delaye; Andrés Moya; Fernando de la Cruz
Journal:  J Bacteriol       Date:  2014-02-07       Impact factor: 3.490

  1 in total

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