| Literature DB >> 12834961 |
B P Matselyukh1, S M Yarmoluk, A B Matselyukh, V B Kovalska, I O Kocheshev, D V Kryvorotenko, S S Lukashov.
Abstract
Fifteen polymethine cyanine dyes were studied as fluorescent stains for DNA in electrophoretic gels. Among studied cyanines, two dyes CPent V and CCyan 2-O most effectively visualized covalently closed and linear double-stranded DNA molecules in gels under standard conditions using UV-illumination, green filter and black-and-white photo film. Ethidium bromide was 1.2-1.6 times more effective as compared to cyanine dyes in staining of DNA in the concentration range of 8-18 ng, while studied cyanines were more sensitive to DNA quantity above 50 ng.Entities:
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Year: 2003 PMID： 12834961 DOI： 10.1016/s0165-022x(03)00036-8
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X