Meal patterns of lean and leptin-deficient obese mice in a simulated foraging environment.

C57BL/6J lean and obese (lep -/-) mice were studied in a closed economy operant protocol that simulates foraging. A predetermined number of presses on a procurement lever (PFR) activated a consumatory lever on which presses would produce 20-mg food pellets. Mice could eat as much as they wished but, once no responding occurred for an elapsed 10-min period, the consumatory lever was inactivated and the procurement or foraging cycle began again. Under these conditions, as has been shown for rats and other species, mice initiated relatively discrete meals (about nine per day) at the lowest PFR, and the number of meals initiated per day decreased with increasing PFR. Meal size increased reciprocally, so that total intake was conserved across the range of PFR examined. Obese mice ate larger meals than lean mice at low PFR, and showed further increases but only at the highest PFRs. The small and inconsistent literature on meal patterns in mice is reviewed, and we discuss the utility of the present protocol to study the interactions between genetic and environmental economic factors, and their implications for the etiology of human obesity.