Literature DB >> 12828947

In vitro analysis of RNA interference in Drosophila melanogaster.

Benjamin Haley1, Guiliang Tang, Phillip D Zamore.   

Abstract

Double-stranded RNA (dsRNA) triggers the destruction of mRNA sharing sequence with the dsRNA, a phenomenon termed RNA interference (RNAi). The dsRNA is converted by endonucleolytic cleavage into 21- to 23-nt small interfering RNAs (siRNAs), which direct a multiprotein complex, the RNA-induced silencing complex to cleave RNA complementary to the siRNA. RNAi can be recapitulated in vitro in lysates of syncytial blastoderm Drosophila embryos. These lysates reproduce all of the known steps in the RNAi pathway in flies and mammals. Here we explain how to prepare and use Drosophila embryo lysates to dissect the mechanism of RNAi.

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Year:  2003        PMID： 12828947     DOI： 10.1016/s1046-2023(03)00052-5

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  56 in total

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5.  Effects of local mRNA structure on posttranscriptional gene silencing.

Authors:  Stephen I Rudnick; Jyothishmathi Swaminathan; Marina Sumaroka; Stephen Liebhaber; Alan M Gewirtz
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6.  Multilayer checkpoints for microRNA authenticity during RISC assembly.

Authors:  Tomoko Kawamata; Mayuko Yoda; Yukihide Tomari
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7.  Noncoding flavivirus RNA displays RNA interference suppressor activity in insect and Mammalian cells.

Authors:  Esther Schnettler; Mark G Sterken; Jason Y Leung; Stefan W Metz; Corinne Geertsema; Rob W Goldbach; Just M Vlak; Alain Kohl; Alexander A Khromykh; Gorben P Pijlman
Journal:  J Virol       Date:  2012-10-03       Impact factor: 5.103

8.  The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster.

Authors:  Ronald P van Rij; Maria-Carla Saleh; Bassam Berry; Catherine Foo; Andrew Houk; Christophe Antoniewski; Raul Andino
Journal:  Genes Dev       Date:  2006-11-01       Impact factor: 11.361

9.  Grafting on a Non-Transgenic Tolerant Tomato Variety Confers Resistance to the Infection of a Sw5-Breaking Strain of Tomato spotted wilt virus via RNA Silencing.

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10.  Suppression of RNA interference increases alphavirus replication and virus-associated mortality in Aedes aegypti mosquitoes.

Authors:  Chris M Cirimotich; Jaclyn C Scott; Aaron T Phillips; Brian J Geiss; Ken E Olson
Journal:  BMC Microbiol       Date:  2009-03-05       Impact factor: 3.605
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