| Literature DB >> 12826059 |
Ren Daming1, Wang Yinyu, Wang Zilai, Cui Jun, Lan Hekui, Zhou Jingye.
Abstract
The complete nucleotide sequence of two cryptic plasmids isolated from Lactobacillus plantarum strain AS1.2986 has been determined. The smaller plasmid, designated pLP2000, encodes a 37.0kDa Rep protein and has a 17bp sequence repeated 10 times. Sequence analysis of the larger plasmid, designated pLP9000, revealed nine putative open reading frames (ORFs). Based on sequence similarity, ORF1 codes for a putative magnesium transporter protein that shows similarities to CorA from plasmid pCIS3 (Lactococcus lactis). None of the nine ORFs shows similarity to any known Rep protein. Southern blot analysis indicates these two plasmids both replicate via a rolling circle (RC) mechanism.Entities:
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Year: 2003 PMID： 12826059 DOI： 10.1016/s0147-619x(03)00010-6
Source DB: PubMed Journal: Plasmid ISSN： 0147-619X Impact factor: 3.466