| Literature DB >> 1282066 |
J Singer-Sam1, J M LeBon, A Dai, A D Riggs.
Abstract
We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript.Mesh：
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Year: 1992 PMID： 1282066 DOI： 10.1101/gr.1.3.160
Source DB: PubMed Journal: PCR Methods Appl ISSN： 1054-9803