Literature DB >> 12814893

Analysis by pulsed-field gel electrophoresis of the genetic diversity among Clostridium perfringens isolates from chickens.

B Nauerby1, K Pedersen, M Madsen.   

Abstract

The aim of this study was to analyse the genetic diversity among Clostridium perfringens isolates from Danish broiler chickens since both sick and presumably healthy animals were investigated. Isolates (n=279) collected from chickens from 25 farms were analysed by pulsed-field gel electrophoresis (PFGE) with the restriction enzyme SmaI. A high genetic diversity was found. Isolates with different PFGE types were toxin typed by PCR and all were found to be of type A. The results showed that healthy broiler chickens carried several different C. perfringens clones both within a flock and even within individual birds, whereas flocks suffering from necrotic enteritis (NE) or cholangio-hepatitis carried only one or two clones.

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Year:  2003        PMID: 12814893     DOI: 10.1016/s0378-1135(03)00118-4

Source DB:  PubMed          Journal:  Vet Microbiol        ISSN: 0378-1135            Impact factor:   3.293


  20 in total

1.  Identification of accessory genome regions in poultry Clostridium perfringens isolates carrying the netB plasmid.

Authors:  D Lepp; J Gong; J G Songer; P Boerlin; V R Parreira; J F Prescott
Journal:  J Bacteriol       Date:  2013-01-04       Impact factor: 3.490

2.  Benefits of Supplementation of an Electrolyte Scour Treatment with a Bacillus-Based Direct-Fed Microbial for Calves.

Authors:  C A Wehnes; K N Novak; V Patskevich; D R Shields; J A Coalson; A H Smith; M E Davis; T G Rehberger
Journal:  Probiotics Antimicrob Proteins       Date:  2009-01-27       Impact factor: 4.609

3.  Conjugation-Mediated Horizontal Gene Transfer of Clostridium perfringens Plasmids in the Chicken Gastrointestinal Tract Results in the Formation of New Virulent Strains.

Authors:  Jake A Lacey; Anthony L Keyburn; Mark E Ford; Ricardo W Portela; Priscilla A Johanesen; Dena Lyras; Robert J Moore
Journal:  Appl Environ Microbiol       Date:  2017-12-01       Impact factor: 4.792

4.  Prevalence and diversity of toxigenic Clostridium perfringens and Clostridium difficile among swine herds in the midwest.

Authors:  Ashley A Baker; Ellen Davis; Thomas Rehberger; Daniel Rosener
Journal:  Appl Environ Microbiol       Date:  2010-03-05       Impact factor: 4.792

5.  Alpha-toxin of Clostridium perfringens is not an essential virulence factor in necrotic enteritis in chickens.

Authors:  Anthony L Keyburn; Scott A Sheedy; Mark E Ford; Mark M Williamson; Milena M Awad; Julian I Rood; Robert J Moore
Journal:  Infect Immun       Date:  2006-08-21       Impact factor: 3.441

6.  Molecular subtyping of poultry-associated type A Clostridium perfringens isolates by repetitive-element PCR.

Authors:  G R Siragusa; M D Danyluk; K L Hiett; M G Wise; S E Craven
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

7.  Multilocus sequence typing analysis of Clostridium perfringens isolates from necrotic enteritis outbreaks in broiler chicken populations.

Authors:  G Chalmers; H L Bruce; D B Hunter; V R Parreira; R R Kulkarni; Y-F Jiang; J F Prescott; P Boerlin
Journal:  J Clin Microbiol       Date:  2008-10-22       Impact factor: 5.948

Review 8.  NetB, a pore-forming toxin from necrotic enteritis strains of Clostridium perfringens.

Authors:  Anthony L Keyburn; Trudi L Bannam; Robert J Moore; Julian I Rood
Journal:  Toxins (Basel)       Date:  2010-07-23       Impact factor: 4.546

9.  Association between avian necrotic enteritis and Clostridium perfringens strains expressing NetB toxin.

Authors:  Anthony L Keyburn; Xu-Xia Yan; Trudi L Bannam; Filip Van Immerseel; Julian I Rood; Robert J Moore
Journal:  Vet Res       Date:  2009-11-25       Impact factor: 3.683

10.  Effective Microorganisms (EM) Improve Internal Organ Morphology, Intestinal Morphometry and Serum Biochemical Activity in Japanese Quails under Clostridium perfringens Challenge.

Authors:  Korina Michalska; Michał Gesek; Rajmund Sokół; Daria Murawska; Mateusz Mikiewicz; Agnieszka Chłodowska
Journal:  Molecules       Date:  2021-05-08       Impact factor: 4.411

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