| Literature DB >> 12805582 |
Yanmin Zhu1, Jaesung Nam, Jaime M Humara, Kirankumar S Mysore, Lan-Ying Lee, Hongbin Cao, Lisa Valentine, Jingling Li, Anthony D Kaiser, Andrea L Kopecky, Hau-Hsuan Hwang, Saikat Bhattacharjee, Praveen K Rao, Tzvi Tzfira, Jyothi Rajagopal, HoChul Yi, Badam S Yadav, Yan M Crane, Kui Lin, Yves Larcher, Matthew J K Gelvin, Marnie Knue, Cynthia Ramos, Xiaowen Zhao, Susan J Davis, Sang-Ic Kim, C T Ranjith-Kumar, Yoo-Jin Choi, Vipin K Hallan, Sudip Chattopadhyay, Xiangzhen Sui, Alicja Ziemienowicz, Ann G Matthysse, Vitaly Citovsky, Barbara Hohn, Stanton B Gelvin.
Abstract
Limited knowledge currently exists regarding the roles of plant genes and proteins in the Agrobacterium tumefaciens-mediated transformation process. To understand the host contribution to transformation, we carried out root-based transformation assays to identify Arabidopsis mutants that are resistant to Agrobacterium transformation (rat mutants). To date, we have identified 126 rat mutants by screening libraries of T-DNA insertion mutants and by using various "reverse genetic" approaches. These mutants disrupt expression of genes of numerous categories, including chromatin structural and remodeling genes, and genes encoding proteins implicated in nuclear targeting, cell wall structure and metabolism, cytoskeleton structure and function, and signal transduction. Here, we present an update on the identification and characterization of these rat mutants.Entities:
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Year: 2003 PMID： 12805582 PMCID： PMC166992 DOI： 10.1104/pp.103.020420
Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340