Venkata Rami K Reddy1, Shrimali K Rajeev, Vijayalakshmi Gupta. 1. Immunology Laboratory, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, India. shrichi@rediffmail.com
Abstract
OBJECTIVE: To determine the clinical significance of alpha6beta1 integrin in human spermatozoa. DESIGN: Prospective case-controlled study. SETTINGS: Hospital infertility clinic in India. PATIENT(S): Sixty-two men undergoing evaluation for infertility and 20 healthy, proven-fertile controls were selected. INTERVENTION(S): Role of alpha6beta1integrin in sperm quality evaluation was determined using semen analysis, cell ELISA, flow cytometry, and in vitro sperm-egg binding. MAIN OUTCOME MEASURE(S): Determination of sperm-associated alpha6beta1 integrin may help in assessing the quality of the human spermatozoa. RESULT(S): Expression of alpha6beta1 integrin was significantly low in the spermatozoa obtained from subfertile men. Only 35%-40% of spermatozoa from fertile men showed a positive reaction to alpha6beta1 antibody. The samples with high rate of acrosomal reaction showed a good expression of alpha6beta1 and maximum binding to oocyte in an in vitro binding assay. CONCLUSION(S): alpha6beta1 Integrin may be used as a clinical marker to evaluate sperm quality.
OBJECTIVE: To determine the clinical significance of alpha6beta1 integrin in human spermatozoa. DESIGN: Prospective case-controlled study. SETTINGS: Hospital infertility clinic in India. PATIENT(S): Sixty-two men undergoing evaluation for infertility and 20 healthy, proven-fertile controls were selected. INTERVENTION(S): Role of alpha6beta1integrin in sperm quality evaluation was determined using semen analysis, cell ELISA, flow cytometry, and in vitro sperm-egg binding. MAIN OUTCOME MEASURE(S): Determination of sperm-associated alpha6beta1 integrin may help in assessing the quality of the human spermatozoa. RESULT(S): Expression of alpha6beta1 integrin was significantly low in the spermatozoa obtained from subfertile men. Only 35%-40% of spermatozoa from fertile men showed a positive reaction to alpha6beta1 antibody. The samples with high rate of acrosomal reaction showed a good expression of alpha6beta1 and maximum binding to oocyte in an in vitro binding assay. CONCLUSION(S): alpha6beta1 Integrin may be used as a clinical marker to evaluate sperm quality.
Authors: Erica T Y Leung; Cheuk-Lun Lee; Xinyi Tian; Kevin K W Lam; Raymond H W Li; Ernest H Y Ng; William S B Yeung; Philip C N Chiu Journal: Nat Rev Urol Date: 2021-11-05 Impact factor: 14.432
Authors: J Jankovicova; M Frolikova; V Palenikova; E Valaskova; J Cerny; P Secova; M Bartokova; L Horovska; P Manaskova-Postlerova; J Antalikova; K Komrskova Journal: Sci Rep Date: 2020-03-09 Impact factor: 4.379