| Literature DB >> 1280113 |
R G Maatman1, E M van de Westerlo, T H van Kuppevelt, J H Veerkamp.
Abstract
The cDNAs of two types of fatty acid-binding protein (FABP) present in human kidney, previously described as types A and B, were isolated using reverse transcriptase-PCR (RT-PCR) with human kidney mRNA and various sets of primers. The cDNA fragments were cloned and sequenced. Renal FABP type A and B cDNAs appeared to be completely identical to human liver- and heart-type FABP cDNAs respectively. In the second part of this study we demonstrated the presence of liver-type FABP in rat kidney by chromatography, e.l.i.s.a. and immunocytochemistry. The ratio and cellular distribution of the two FABP types varies markedly in human and rat kidney. Using RT-PCR we were also able to prepare and identify liver- and heart-type FABP cDNAs with mRNA from both male and female rat kidney.Entities:
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Year: 1992 PMID： 1280113 PMCID： PMC1132111 DOI： 10.1042/bj2880285
Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857