Literature DB >> 2462524

Mechanisms underlying generation of gradients in gene expression within the intestine: an analysis using transgenic mice containing fatty acid binding protein-human growth hormone fusion genes.

D A Sweetser1, E H Birkenmeier, P C Hoppe, D W McKeel, J I Gordon.   

Abstract

The intestine is lined by a continuously regenerating epithelium that maintains gradients in 'liver' fatty acid binding protein (L-FABP) gene expression along its horizontal and vertical axes, i.e., from duodenum to colon and from crypt to villus tip. To identify cis-acting DNA sequences responsible for these regional differences, we linked portions of the L-FABP gene's 5' nontranscribed region to the human growth hormone (hGH) gene and examined hGH expression in transgenic mice. Nucleotides -596 to +21 of the rat L-FABP gene correctly directed hGH expression to enterocytes and hepatocytes. However, anomalous expression was observed in small intestinal crypts, colon, and renal proximal tubular epithelial cells. Addition of nucleotides -4000 to -597 of the L-FABP gene, in either orientation, suppressed renal hGH expression and restored a nearly normal horizontal, but not a vertical, hGH gradient in the intestine. Thus, horizontal gradients of gene expression within the intestine can be maintained by orientation-independent, cis-acting suppressor elements.

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Year:  1988        PMID: 2462524     DOI: 10.1101/gad.2.10.1318

Source DB:  PubMed          Journal:  Genes Dev        ISSN: 0890-9369            Impact factor:   11.361


  48 in total

1.  PDX1 regulation of FABP1 and novel target genes in human intestinal epithelial Caco-2 cells.

Authors:  Chin Chen; Rixun Fang; Lin-Chiang Chou; Anson W Lowe; Eric Sibley
Journal:  Biochem Biophys Res Commun       Date:  2012-05-26       Impact factor: 3.575

Review 2.  Fatty acid-binding protein expression in the liver: its regulation and relationship to the zonation of fatty acid metabolism.

Authors:  N M Bass
Journal:  Mol Cell Biochem       Date:  1990 Oct 15-Nov 8       Impact factor: 3.396

Review 3.  Historic overview of studies on fatty acid-binding proteins.

Authors:  R K Ockner
Journal:  Mol Cell Biochem       Date:  1990 Oct 15-Nov 8       Impact factor: 3.396

Review 4.  Cellular fatty acid-binding proteins: current concepts and future directions.

Authors:  J F Glatz; G J van der Vusse
Journal:  Mol Cell Biochem       Date:  1990 Oct 15-Nov 8       Impact factor: 3.396

5.  Two types of fatty acid-binding protein in human kidney. Isolation, characterization and localization.

Authors:  R G Maatman; T H Van Kuppevelt; J H Veerkamp
Journal:  Biochem J       Date:  1991-02-01       Impact factor: 3.857

6.  Specific growth stimulation by linoleic acid in hepatoma cell lines transfected with the target protein of a liver carcinogen.

Authors:  T Keler; C S Barker; S Sorof
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-01       Impact factor: 11.205

Review 7.  Functions of fatty acid binding proteins.

Authors:  R M Kaikaus; N M Bass; R K Ockner
Journal:  Experientia       Date:  1990-06-15

8.  High-level expression of the rat whey acidic protein gene is mediated by elements in the promoter and 3' untranslated region.

Authors:  T C Dale; M J Krnacik; C Schmidhauser; C L Yang; M J Bissell; J M Rosen
Journal:  Mol Cell Biol       Date:  1992-03       Impact factor: 4.272

9.  Liver fatty acid-binding protein: specific mediator of the mitogenesis induced by two classes of carcinogenic peroxisome proliferators.

Authors:  S H Khan; S Sorof
Journal:  Proc Natl Acad Sci U S A       Date:  1994-02-01       Impact factor: 11.205

10.  Response of small intestinal epithelial cells to acute disruption of cell division through CDC25 deletion.

Authors:  Gwanghee Lee; Lynn S White; Kristen E Hurov; Thaddeus S Stappenbeck; Helen Piwnica-Worms
Journal:  Proc Natl Acad Sci U S A       Date:  2009-03-09       Impact factor: 11.205

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