Cunping Qiu1, Hong Wu, Huajun He, Weiliu Qiu. 1. Visiting Fellow, Laboratory of Experimental Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. cunping_qui@nih.gov
Abstract
PURPOSE: A lymph node metastatic model of human tongue carcinoma using orthotopic and serial transplantation was established in nude mice to study the invasive and metastatic properties of human tongue cancer. MATERIALS AND METHODS: Lymph node metastatic specimens of human tongue carcinoma were transplanted into nude mice orthotopically. Tumors dissected from the metastatic lymph nodes of the nude mice were serially transplanted into tongues of disease-free nude mice at 4-week intervals. RESULTS: All mice developed aggressive and diffuse well-differentiated squamous cell carcinoma at tongue recipient sites. Tumor cells invaded to lymphatic vessels. In addition, increased cervical lymph node metastasis was noted in the first (3 of 14, or 21%), second (4 of 11, or 36%), third (6 of 10, or 60%), or fourth (11 of 14, or 79%) transplantation. In mice, 2 of 14 lung metastases were found in the fourth round of transplantation. CONCLUSION: After surgical specimens of the lymph node metastasis for human tongue cancer were transplanted into the tongue of nude mice, the clinical characteristics of human tongue carcinoma, especially invasion and metastasis, were observed. This metastatic model involving orthotopic and serial transplantation should be useful for studies on the mechanisms, treatment, and prevention of human carcinoma of tongue. Copyright 2003 American Association of Oral and Maxillofacial Surgeons J Oral Maxillofac Surg 61:696-700, 2003
PURPOSE: A lymph node metastatic model of humantongue carcinoma using orthotopic and serial transplantation was established in nude mice to study the invasive and metastatic properties of humantongue cancer. MATERIALS AND METHODS: Lymph node metastatic specimens of humantongue carcinoma were transplanted into nude mice orthotopically. Tumors dissected from the metastatic lymph nodes of the nude mice were serially transplanted into tongues of disease-free nude mice at 4-week intervals. RESULTS: All mice developed aggressive and diffuse well-differentiated squamous cell carcinoma at tongue recipient sites. Tumor cells invaded to lymphatic vessels. In addition, increased cervical lymph node metastasis was noted in the first (3 of 14, or 21%), second (4 of 11, or 36%), third (6 of 10, or 60%), or fourth (11 of 14, or 79%) transplantation. In mice, 2 of 14 lung metastases were found in the fourth round of transplantation. CONCLUSION: After surgical specimens of the lymph node metastasis for humantongue cancer were transplanted into the tongue of nude mice, the clinical characteristics of humantongue carcinoma, especially invasion and metastasis, were observed. This metastatic model involving orthotopic and serial transplantation should be useful for studies on the mechanisms, treatment, and prevention of humancarcinoma of tongue. Copyright 2003 American Association of Oral and Maxillofacial Surgeons J Oral Maxillofac Surg 61:696-700, 2003
Authors: Andrew P Stein; Sandeep Saha; Cheng Z Liu; Gregory K Hartig; Paul F Lambert; Randall J Kimple Journal: PLoS One Date: 2014-06-26 Impact factor: 3.240