| Literature DB >> 12796781 |
Alan J Bridge1, Stephanie Pebernard, Annick Ducraux, Anne-Laure Nicoulaz, Richard Iggo.
Abstract
DNA vectors that express short hairpin RNAs (shRNAs) from RNA polymerase III (Pol III) promoters are a promising new tool to reduce gene expression in mammalian cells. shRNAs are processed to small interfering RNAs (siRNAs) of 21 nucleotides (nt) that guide the cleavage of the cognate mRNA by the RNA-induced silencing complex. Although siRNAs are thought to be too short to induce interferon expression, we report here that a substantial number of shRNA vectors can trigger an interferon response.Entities:
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Year: 2003 PMID： 12796781 DOI： 10.1038/ng1173
Source DB: PubMed Journal: Nat Genet ISSN： 1061-4036 Impact factor: 38.330