Literature DB >> 12791900

Multiplex PCR assay for identification of human diarrheagenic Escherichia coli.

Claudia Toma1, Yan Lu, Naomi Higa, Noboru Nakasone, Isabel Chinen, Ariela Baschkier, Marta Rivas, Masaaki Iwanaga.   

Abstract

A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli strain in a single reaction tube.

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Year:  2003        PMID： 12791900      PMCID： PMC156568          DOI： 10.1128/JCM.41.6.2669-2671.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

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  58 in total

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6.  Rapid identification and differentiation of clinical isolates of enteropathogenic Escherichia coli (EPEC), atypical EPEC, and Shiga toxin-producing Escherichia coli by a one-step multiplex PCR method.

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Authors:  Octaviana Baccin Fialho; Emanuel Maltempi de Souza; Cibelle de Borba Dallagassa; Fábio de Oliveira Pedrosa; Giseli Klassen; Kinue Irino; Katia Sabrina Paludo; Flávia Emanoelli Araújo de Assis; Monica Surek; Sônia Maria de Souza Santos Farah; Cyntia Maria Telles Fadel-Picheth
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10.  Comparison between O serotyping method and multiplex real-time PCR to identify diarrheagenic Escherichia coli in Taiwan.

Authors:  Ji-Rong Yang; Fang-Tzy Wu; Jin-Lai Tsai; Jung-Jung Mu; Ling-Fen Lin; Kuang-Lo Chen; Steve Hsu-Sung Kuo; Chuen-Sheue Chiang; Ho-Sheng Wu
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