Literature DB >> 16825399

Rapid identification and differentiation of clinical isolates of enteropathogenic Escherichia coli (EPEC), atypical EPEC, and Shiga toxin-producing Escherichia coli by a one-step multiplex PCR method.

Daniel Müller1, Peter Hagedorn, Sabine Brast, Gerhard Heusipp, Martina Bielaszewska, Alexander W Friedrich, Helge Karch, M Alexander Schmidt.   

Abstract

Enteropathogenic Escherichia coli (EPEC), atypical enteropathogenic E. coli, and Shiga toxin-producing E. coli differ in their virulence factor profiles, clinical manifestations, and prognosis, and they require different therapeutic measures. We developed and evaluated a robust multiplex PCR to identify these pathogroups based on sequences complementary to escV, bfpB, stx1, and stx2.

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Year:  2006        PMID： 16825399      PMCID： PMC1489516          DOI： 10.1128/JCM.00895-06

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  30 in total

1.  A rapid method for the discrimination of genes encoding classical Shiga toxin (Stx) 1 and its variants, Stx1c and Stx1d, in Escherichia coli.

Authors:  Thorsten Kuczius; Martina Bielaszewska; Alexander W Friedrich; Wenlan Zhang
Journal:  Mol Nutr Food Res       Date:  2004-12       Impact factor: 5.914

Review 2.  Target genes for virulence assessment of Escherichia coli isolates from water, food and the environment.

Authors:  P Kuhnert; P Boerlin; J Frey
Journal:  FEMS Microbiol Rev       Date:  2000-01       Impact factor: 16.408

3.  Diffusely adhering Escherichia coli strains induce attaching and effacing phenotypes and secrete homologs of Esp proteins.

Authors:  C Beinke; S Laarmann; C Wachter; H Karch; L Greune; M A Schmidt
Journal:  Infect Immun       Date:  1998-02       Impact factor: 3.441

4.  Evaluation of multiplex PCRs for diagnosis of infection with diarrheagenic Escherichia coli and Shigella spp.

Authors:  K R S Aranda; U Fagundes-Neto; I C A Scaletsky
Journal:  J Clin Microbiol       Date:  2004-12       Impact factor: 5.948

5.  A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons.

Authors:  H Schmidt; J Scheef; S Morabito; A Caprioli; L H Wieler; H Karch
Journal:  Appl Environ Microbiol       Date:  2000-03       Impact factor: 4.792

6.  Development of PCR for screening of enteroaggregative Escherichia coli.

Authors:  H Schmidt; C Knop; S Franke; S Aleksic; J Heesemann; H Karch
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948

7.  Multiplex PCR-DNA probe assay for the detection of pathogenic Escherichia coli.

Authors:  Leigh Watterworth; Edward Topp; Heidi Schraft; Kam Tin Leung
Journal:  J Microbiol Methods       Date:  2005-01       Impact factor: 2.363

8.  Escherichia coli and community-acquired gastroenteritis, Melbourne, Australia.

Authors:  Roy M Robins-Browne; Anne-Marie Bordun; Marija Tauschek; Vicki R Bennett-Wood; Jacinta Russell; Frances Oppedisano; Nicole A Lister; Karl A Bettelheim; Christopher K Fairley; Martha I Sinclair; Margaret E Hellard
Journal:  Emerg Infect Dis       Date:  2004-10       Impact factor: 6.883

9.  Atypical enteropathogenic Escherichia coli infection and prolonged diarrhea in children.

Authors:  Rang N Nguyen; Louise S Taylor; Marija Tauschek; Roy M Robins-Browne
Journal:  Emerg Infect Dis       Date:  2006-04       Impact factor: 6.883

10.  Rapid detection of diarrheagenic E. coli by real-time PCR.

Authors:  C Bischoff; J Lüthy; M Altwegg; F Baggi
Journal:  J Microbiol Methods       Date:  2005-01-11       Impact factor: 2.363
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  12 in total

1.  Comparison of the EntericBio multiplex PCR system with routine culture for detection of bacterial enteric pathogens.

Authors:  James O'Leary; Daniel Corcoran; Brigid Lucey
Journal:  J Clin Microbiol       Date:  2009-09-02       Impact factor: 5.948

2.  Analysis of Escherichia coli isolated from patients affected by Crohn's disease.

Authors:  Tiziana Raso; Sabrina Crivellaro; Maria Gabriella Chirillo; Paolo Pais; Ezio Gaia; Dianella Savoia
Journal:  Curr Microbiol       Date:  2011-05-28       Impact factor: 2.188

3.  Identification of unconventional intestinal pathogenic Escherichia coli isolates expressing intermediate virulence factor profiles by using a novel single-step multiplex PCR.

Authors:  Daniel Müller; Lilo Greune; Gerhard Heusipp; Helge Karch; Angelika Fruth; Helmut Tschäpe; M Alexander Schmidt
Journal:  Appl Environ Microbiol       Date:  2007-03-30       Impact factor: 4.792

Review 4.  Enteropathogenic escherichia coli infection in children.

Authors:  Theresa J Ochoa; Carmen A Contreras
Journal:  Curr Opin Infect Dis       Date:  2011-10       Impact factor: 4.915

5.  Norwegian sheep are an important reservoir for human-pathogenic Escherichia coli O26:H11.

Authors:  Lin T Brandal; Camilla Sekse; Bjørn-Arne Lindstedt; Marianne Sunde; Inger Løbersli; Anne Margrete Urdahl; Georg Kapperud
Journal:  Appl Environ Microbiol       Date:  2012-04-06       Impact factor: 4.792

6.  Characterization of Salmonella spp. and E. coli Strains Isolated from Wild Carnivores in Janos Biosphere Reserve, Mexico.

Authors:  Jonathan J López-Islas; Estela T Méndez-Olvera; Daniel Martínez-Gómez; Andrés M López-Pérez; Libertad Orozco; Gerardo Suzan; Carlos Eslava
Journal:  Animals (Basel)       Date:  2022-04-20       Impact factor: 3.231

7.  Intestine and environment of the chicken as reservoirs for extraintestinal pathogenic Escherichia coli strains with zoonotic potential.

Authors:  Christa Ewers; Esther-Maria Antão; Ines Diehl; Hans-C Philipp; Lothar H Wieler
Journal:  Appl Environ Microbiol       Date:  2008-11-07       Impact factor: 4.792

8.  Characterisation of atypical enteropathogenic E. coli strains of clinical origin.

Authors:  Sharon M Tennant; Marija Tauschek; Kristy Azzopardi; Andrea Bigham; Vicki Bennett-Wood; Elizabeth L Hartland; Weihong Qi; Thomas S Whittam; Roy M Robins-Browne
Journal:  BMC Microbiol       Date:  2009-06-03       Impact factor: 3.605

9.  Shiga toxin, cytolethal distending toxin, and hemolysin repertoires in clinical Escherichia coli O91 isolates.

Authors:  Martina Bielaszewska; Franziska Stoewe; Angelika Fruth; Wenlan Zhang; Rita Prager; Jens Brockmeyer; Alexander Mellmann; Helge Karch; Alexander W Friedrich
Journal:  J Clin Microbiol       Date:  2009-04-29       Impact factor: 5.948

10.  Prevalence of virulence determinants and antimicrobial resistance among commensal Escherichia coli derived from dairy and beef cattle.

Authors:  Ewa Bok; Justyna Mazurek; Michał Stosik; Magdalena Wojciech; Katarzyna Baldy-Chudzik
Journal:  Int J Environ Res Public Health       Date:  2015-01-19       Impact factor: 3.390
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