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Literature DB >> 12787667

Role of E.coli transcription-repair coupling factor Mfd in Nun-mediated transcription termination.

Robert S Washburn¹, Yousong Wang, Max E Gottesman.

Abstract

Phage HK022 Nun protein excludes phage lambda by binding nascent lambda-nut RNA and inducing termination and transcript release. In contrast, in a purified in vitro system, Nun arrests transcription on lambdaDNA templates without dissociation of the transcription elongation complex (TEC). Our evidence indicates that transcription-repair coupling factor (Mfd) frees Nun-arrested RNA polymerase. The activity of Nun is enhanced in an mfd-null mutant, consistent with prolonged association of Nun with the TEC. Furthermore, expression of lambda nut RNA in the mfd mutant titrates Nun, allowing superinfecting lambda to form plaques. Finally, addition of Mfd releases a Nun-arrested transcription complex in vitro.

Entities: Gene Species

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Year: 2003 PMID： 12787667 DOI： 10.1016/s0022-2836(03)00465-0

Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469

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Cited

27 in total

1. A DNA translocation motif in the bacterial transcription--repair coupling factor, Mfd.

Authors: A L Chambers; A J Smith; N J Savery
Journal: Nucleic Acids Res Date: 2003-11-15 Impact factor: 16.971

2. Nucleotide excision repair (NER) machinery recruitment by the transcription-repair coupling factor involves unmasking of a conserved intramolecular interface.

Authors: Alexandra M Deaconescu; Anastasia Sevostyanova; Irina Artsimovitch; Nikolaus Grigorieff
Journal: Proc Natl Acad Sci U S A Date: 2012-02-13 Impact factor: 11.205

3. Transcription termination maintains chromosome integrity.

Authors: Robert S Washburn; Max E Gottesman
Journal: Proc Natl Acad Sci U S A Date: 2010-12-23 Impact factor: 11.205

Review 4. RNA polymerase elongation factors.

Authors: Jeffrey W Roberts; Smita Shankar; Joshua J Filter
Journal: Annu Rev Microbiol Date: 2008 Impact factor: 15.500

Review 5. Transcription elongation.

Authors: Arkady Mustaev; Jeffrey Roberts; Max Gottesman
Journal: Transcription Date: 2017-02-08

Role of E.coli transcription-repair coupling factor Mfd in Nun-mediated transcription termination.

1. A DNA translocation motif in the bacterial transcription--repair coupling factor, Mfd.

2. Nucleotide excision repair (NER) machinery recruitment by the transcription-repair coupling factor involves unmasking of a conserved intramolecular interface.

3. Transcription termination maintains chromosome integrity.

Review 4. RNA polymerase elongation factors.

Review 5. Transcription elongation.

6. Role of an RNase III binding site in transcription termination at lambda nutL by HK022 Nun protein.

7. Linking RNA polymerase backtracking to genome instability in E. coli.

Review 8. Mfd Protein and Transcription-Repair Coupling in Escherichia coli.

9. Roadblock repression of transcription by Bacillus subtilis CodY.

10. Termination factor Rho and its cofactors NusA and NusG silence foreign DNA in E. coli.