Literature DB >> 12767997

A transcriptionally active subgenomic promoter supports homologous crossovers in a plus-strand RNA virus.

Rafal Wierzchoslawski1, Aleksandra Dzianott, Selvi Kunimalayan, Jozef J Bujarski.   

Abstract

Genetic RNA recombination plays an important role in viral evolution, but its molecular mechanism is not well understood. In this work we describe homologous RNA recombination activity that is supported by a subgenomic promoter (sgp) region in the RNA3 segment of brome mosaic bromovirus (BMV), a tripartite plus-strand RNA virus. The crossover frequencies were determined by coinoculations with pairs of BMV RNA3 variants that carried a duplicated sgp region flanked by marker restriction sites. A region composed of the sgp core, a poly(A) tract, and an upstream enhancer supported homologous exchanges in 25% of the analyzed RNA3 progeny. However, mutations in the sgp core stopped both the transcription of the sgp RNA and homologous recombination. These data provide evidence for an association of RNA recombination with transcription.

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Year:  2003        PMID： 12767997      PMCID： PMC156210          DOI： 10.1128/jvi.77.12.6769-6776.2003

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  33 in total

1.  Factors regulating template switch in vitro by viral RNA-dependent RNA polymerases: implications for RNA-RNA recombination.

Authors:  M J Kim; C Kao
Journal:  Proc Natl Acad Sci U S A       Date:  2001-04-17       Impact factor: 11.205

Review 2.  Synthesis of subgenomic RNAs by positive-strand RNA viruses.

Authors:  W A Miller; G Koev
Journal:  Virology       Date:  2000-07-20       Impact factor: 3.616

3.  Studies on functional interaction between brome mosaic virus replicase proteins during RNA recombination, using combined mutants in vivo and in vitro.

Authors:  A Dzianott; N Rauffer-Bruyere; J J Bujarski
Journal:  Virology       Date:  2001-10-10       Impact factor: 3.616

4.  Sequence-specific recognition of a subgenomic RNA promoter by a viral RNA polymerase.

Authors:  R W Siegel; S Adkins; C C Kao
Journal:  Proc Natl Acad Sci U S A       Date:  1997-10-14       Impact factor: 11.205

Review 5.  RNA recombination in brome mosaic virus, a model plus strand RNA virus.

Authors:  M Figlerowicz; J J Bujarski
Journal:  Acta Biochim Pol       Date:  1998       Impact factor: 2.149

6.  Nonreplicative RNA recombination in poliovirus.

Authors:  A P Gmyl; E V Belousov; S V Maslova; E V Khitrina; A B Chetverin; V I Agol
Journal:  J Virol       Date:  1999-11       Impact factor: 5.103

7.  Recombination between viral RNA and transgenic plant transcripts.

Authors:  A E Greene; R F Allison
Journal:  Science       Date:  1994-03-11       Impact factor: 47.728

8.  Efficient system of homologous RNA recombination in brome mosaic virus: sequence and structure requirements and accuracy of crossovers.

Authors:  P D Nagy; J J Bujarski
Journal:  J Virol       Date:  1995-01       Impact factor: 5.103

9.  Enhancer-like activity of a brome mosaic virus RNA promoter.

Authors:  C T Ranjith-Kumar; Xin Zhang; C Cheng Kao
Journal:  J Virol       Date:  2003-02       Impact factor: 5.103

10.  RNA elements required for RNA recombination function as replication enhancers in vitro and in vivo in a plus-strand RNA virus.

Authors:  P D Nagy; J Pogany; A E Simon
Journal:  EMBO J       Date:  1999-10-15       Impact factor: 11.598
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  10 in total

1.  Homologous crossovers among molecules of brome mosaic bromovirus RNA1 or RNA2 segments in vivo.

Authors:  Anna Urbanowicz; Magdalena Alejska; Piotr Formanowicz; Jacek Blazewicz; Marek Figlerowicz; Jozef J Bujarski
Journal:  J Virol       Date:  2005-05       Impact factor: 5.103

2.  Efficient in vitro system of homologous recombination in brome mosaic bromovirus.

Authors:  Rafal Wierzchoslawski; Jozef J Bujarski
Journal:  J Virol       Date:  2006-06       Impact factor: 5.103

3.  Characterization of a novel 5' subgenomic RNA3a derived from RNA3 of Brome mosaic bromovirus.

Authors:  Rafal Wierzchoslawski; Anna Urbanowicz; Aleksandra Dzianott; Marek Figlerowicz; Jozef J Bujarski
Journal:  J Virol       Date:  2006-09-27       Impact factor: 5.103

4.  Dissecting the requirement for subgenomic promoter sequences by RNA recombination of brome mosaic virus in vivo: evidence for functional separation of transcription and recombination.

Authors:  Rafal Wierzchoslawski; Aleksandra Dzianott; Jozef Bujarski
Journal:  J Virol       Date:  2004-08       Impact factor: 5.103

5.  The AU-rich RNA recombination hot spot sequence of Brome mosaic virus is functional in tombusviruses: implications for the mechanism of RNA recombination.

Authors:  Natalia Shapka; Peter D Nagy
Journal:  J Virol       Date:  2004-03       Impact factor: 5.103

6.  Defective Interfering RNAs: Foes of Viruses and Friends of Virologists.

Authors:  Kunj B Pathak; Peter D Nagy
Journal:  Viruses       Date:  2009-11-10       Impact factor: 5.818

7.  Co-infection with two strains of Brome mosaic bromovirus reveals common RNA recombination sites in different hosts.

Authors:  Beivy Kolondam; Parth Rao; Joanna Sztuba-Solinska; Philipp H Weber; Aleksandra Dzianott; Mitrick A Johns; Jozef J Bujarski
Journal:  Virus Evol       Date:  2015-12-23

8.  Phylogenetic and Molecular Variability Studies Reveal a New Genetic Clade of Citrus leprosis virus C.

Authors:  Pedro Luis Ramos-González; Camila Chabi-Jesus; Orlene Guerra-Peraza; Michèle Claire Breton; Gabriella Dias Arena; Maria Andreia Nunes; Elliot Watanabe Kitajima; Marcos Antonio Machado; Juliana Freitas-Astúa
Journal:  Viruses       Date:  2016-06-06       Impact factor: 5.048

9.  Recombination of 5' subgenomic RNA3a with genomic RNA3 of Brome mosaic bromovirus in vitro and in vivo.

Authors:  Joanna Sztuba-Solińska; Aleksandra Dzianott; Jozef J Bujarski
Journal:  Virology       Date:  2010-11-26       Impact factor: 3.616

10.  Intermolecular RNA Recombination Occurs at Different Frequencies in Alternate Forms of Brome Mosaic Virus RNA Replication Compartments.

Authors:  Hernan Garcia-Ruiz; Arturo Diaz; Paul Ahlquist
Journal:  Viruses       Date:  2018-03-15       Impact factor: 5.048
  10 in total

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