Literature DB >> 12766403

Backbone resonance assignment in large protonated proteins using a combination of new 3D TROSY-HN(CA)HA, 4D TROSY-HACANH and 13C-detected HACACO experiments.

Kaifeng Hu1, Alexander Eletsky, Konstantin Pervushin.   

Abstract

A new method for backbone resonance assignment suitable for large proteins with the natural (1)H isotope content is proposed based on a combination of the most sensitive TROSY-type triple-resonance experiments. These techniques include TROSY-HNCO, (13)C'-detected 3D multiple-quantum HACACO and the newly developed 3D TROSY multiple-quantum-HN(CA)HA and 4D TROSY multiple-quantum-HACANH experiments. The favorable relaxation properties of the multiple-quantum coherences, signal detection using the (13)C' antiphase coherences, and the use of TROSY optimize the performance of the proposed set of experiments for application to large protonated proteins. The method is demonstrated with the 44 kDa uniformly (15)N,(13)C-labeled and fractionally (35%) deuterated trimeric B. Subtilis Chorismate Mutase and is suitable for proteins with large correlation times but a relatively small number of residues, such as membrane proteins embedded in micelles or oligomeric proteins.

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Year:  2003        PMID： 12766403     DOI： 10.1023/a:1023008719248

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  17 in total

1.  Evaluation of cross-correlation effects and measurement of one-bond couplings in proteins with short transverse relaxation times.

Authors:  G Kontaxis; G M Clore; A Bax
Journal:  J Magn Reson       Date:  2000-03       Impact factor: 2.229

2.  Assignment of 1H(N), 15N, 13C(alpha), 13CO and 13C(beta) resonances in a 67 kDa p53 dimer using 4D-TROSY NMR spectroscopy.

Authors:  F A Mulder; A Ayed; D Yang; C H Arrowsmith; L E Kay
Journal:  J Biomol NMR       Date:  2000-10       Impact factor: 2.835

3.  Application of multiple-quantum line narrowing with simultaneous 1H and 13C constant-time scalar-coupling evolution in PFG-HACANH and PFG-HACA(CO)NH triple-resonance experiments.

Authors:  G V Swapna; C B Rios; Z Shang; G T Montelione
Journal:  J Biomol NMR       Date:  1997-01       Impact factor: 2.835

4.  The monofunctional chorismate mutase from Bacillus subtilis. Structure determination of chorismate mutase and its complexes with a transition state analog and prephenate, and implications for the mechanism of the enzymatic reaction.

Authors:  Y M Chook; J V Gray; H Ke; W N Lipscomb
Journal:  J Mol Biol       Date:  1994-07-29       Impact factor: 5.469

5.  [13C]-constant-time [15N,1H]-TROSY-HNCA for sequential assignments of large proteins.

Authors:  M Salzmann; K Pervushin; G Wider; H Senn; K Wüthrich
Journal:  J Biomol NMR       Date:  1999-05       Impact factor: 2.835

6.  TROSY NMR with partially deuterated proteins.

Authors:  A Eletsky; A Kienhöfer; K Pervushin
Journal:  J Biomol NMR       Date:  2001-06       Impact factor: 2.835

7.  Single Transition-to-single Transition Polarization Transfer (ST2-PT) in [15N,1H]-TROSY.

Authors:  K V Pervushin; G Wider; K Wüthrich
Journal:  J Biomol NMR       Date:  1998-08       Impact factor: 2.835

8.  Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution.

Authors:  K Pervushin; R Riek; G Wider; K Wüthrich
Journal:  Proc Natl Acad Sci U S A       Date:  1997-11-11       Impact factor: 11.205

9.  Characterizing the use of perdeuteration in NMR studies of large proteins: 13C, 15N and 1H assignments of human carbonic anhydrase II.

Authors:  R A Venters; B T Farmer; C A Fierke; L D Spicer
Journal:  J Mol Biol       Date:  1996-12-20       Impact factor: 5.469

10.  Improved sensitivity and coherence selection for [15N,1H]-TROSY elements in triple resonance experiments.

Authors:  M Salzmann; G Wider; K Pervushin; K Wüthrich
Journal:  J Biomol NMR       Date:  1999-10       Impact factor: 2.835
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  5 in total

1.  Recent Advances in the Application of Solution NMR Spectroscopy to Multi-Span Integral Membrane Proteins.

Authors:  Hak Jun Kim; Stanley C Howell; Wade D Van Horn; Young Ho Jeon; Charles R Sanders
Journal:  Prog Nucl Magn Reson Spectrosc       Date:  2009-11-01       Impact factor: 9.795

2.  13C-detected HN(CA)C and HMCMC experiments using a single methyl-reprotonated sample for unambiguous methyl resonance assignment.

Authors:  Kaifeng Hu; Beat Vögeli; G Marius Clore
Journal:  J Biomol NMR       Date:  2006-10-12       Impact factor: 2.835

3.  Simultaneous 1H- or 2H-, 15N- and multiple-band-selective 13C-decoupling during acquisition in 13C-detected experiments with proteins and oligonucleotides.

Authors:  Beat Vögeli; Helena Kovacs; Konstantin Pervushin
Journal:  J Biomol NMR       Date:  2005-01       Impact factor: 2.835

4.  Assignment of 1H, 13C and 15N backbone resonances of Escherichia coli LpxC bound to L-161,240.

Authors:  Adam W Barb; Ling Jiang; Christian R H Raetz; Pei Zhou
Journal:  Biomol NMR Assign       Date:  2009-11-26       Impact factor: 0.746

Review 5.  13C Direct Detected NMR for Challenging Systems.

Authors:  Isabella C Felli; Roberta Pierattelli
Journal:  Chem Rev       Date:  2022-01-13       Impact factor: 72.087
  5 in total

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