Literature DB >> 12739

Electrophoretic demonstration and inital characterization of human red cell NAD(P)+ase.

R Ravazzolo, G Bruzzone, C Garrè, F Ajmar.   

Abstract

A method for electrophoretic detection of NAD(P)+ase solubilized from red cell membranes is described. The method reveals both NAD+ase and NADP+ase and can be applied to a screening procedure for the detection of electrophoretic variants. The initial chracterization of the solubilized enzyme is also described.

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Year:  1976        PMID： 12739     DOI： 10.1007/BF00485349

Source DB:  PubMed          Journal:  Biochem Genet        ISSN： 0006-2928            Impact factor:   1.890


  9 in total

1.  A simple technique for eliminating interference by detergents in the Lowry method of protein determination.

Authors:  J R Dulley; P A Grieve
Journal:  Anal Biochem       Date:  1975-03       Impact factor: 3.365

2.  The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes.

Authors:  J T DODGE; C MITCHELL; D J HANAHAN
Journal:  Arch Biochem Biophys       Date:  1963-01       Impact factor: 4.013

3.  The metabolism of the erythrocyte. IX. Diphosphopyridine nucleotidase of erythrocytes.

Authors:  S G ALIVISATOS; S KASHKET; O F DENSTEDT
Journal:  Can J Biochem Physiol       Date:  1956-01

4.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

5.  NAD(P) glycohydrolase deficiency in human erythrocytes and alteration of cytosol NADH-methemoglobin diaphorase by membrane NAD-glycohydrolase activity.

Authors:  H Frischer; R Nelson; C Noyes; P E Carson; J E Bowman; K H Rieckmann; F Ajmar
Journal:  Proc Natl Acad Sci U S A       Date:  1973-08       Impact factor: 11.205

6.  A method of localization of soluble NAD-glycohydrolase from mycobacterial extracts after electrophoresis on cellulose acetate.

Authors:  I Flechner; S Hirschhorn; A Bekierkunst
Journal:  Life Sci       Date:  1968-12-15       Impact factor: 5.037

7.  Purification and characterization of diphosphopyridine nucleosidase from pig brain.

Authors:  N I Swislocki; N O Kaplan
Journal:  J Biol Chem       Date:  1967-03-25       Impact factor: 5.157

8.  Deficiency of erythrocyte nicotinamide adenine dinucleotide nucleosidase (NADase) activity in the Negro.

Authors:  W G Ng; G N Donnell; W Bergren
Journal:  Nature       Date:  1968-01-06       Impact factor: 49.962

9.  A simple procedure for removal of Triton X-100 from protein samples.

Authors:  P W Holloway
Journal:  Anal Biochem       Date:  1973-05       Impact factor: 3.365
  9 in total

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