Jian Kong1, Peter Gouras. 1. Department of Ophthalmology, Columbia University, New York, NY 10032, USA.
Abstract
PURPOSE: To study the effect of body temperature on the murine electroretinogram (ERG). METHODS: The corneal ERG elicited by a strobe flash from dark-adapted mice was recorded using a saline wick electrode while measuring rectal temperature continuously. The mouse was placed within a cylindrical coil of tubing through which water circulated from a temperature controlled bath. The body temperature of the mouse was changed stepwise between 30 and 37 degrees C. RESULTS: ERGs of approximately normal configuration were recorded at body temperature ranging between 30 and 37 degrees C. The maximum amplitude of the a- and b-waves varied linearly with temperature. The rate of change of b-wave amplitude was about 100 microV/degree. At 30 degrees C, maximum b-wave amplitude was about 400 microV; at 37 degrees C it was about 1000 microV. A change in body temperature produced a rapid change in ERG amplitude. CONCLUSION: The murine ERG is very sensitive to changes in temperature. In order to monitor the ERG accurately over time, continuous recording of body temperature is essential.
PURPOSE: To study the effect of body temperature on the murine electroretinogram (ERG). METHODS: The corneal ERG elicited by a strobe flash from dark-adapted mice was recorded using a saline wick electrode while measuring rectal temperature continuously. The mouse was placed within a cylindrical coil of tubing through which water circulated from a temperature controlled bath. The body temperature of the mouse was changed stepwise between 30 and 37 degrees C. RESULTS: ERGs of approximately normal configuration were recorded at body temperature ranging between 30 and 37 degrees C. The maximum amplitude of the a- and b-waves varied linearly with temperature. The rate of change of b-wave amplitude was about 100 microV/degree. At 30 degrees C, maximum b-wave amplitude was about 400 microV; at 37 degrees C it was about 1000 microV. A change in body temperature produced a rapid change in ERG amplitude. CONCLUSION: The murine ERG is very sensitive to changes in temperature. In order to monitor the ERG accurately over time, continuous recording of body temperature is essential.
Authors: John R Hetling; Monica S Baig-Silva; Christopher M Comer; Machelle T Pardue; Dalia Y Samaan; Nasser M Qtaishat; David R Pepperberg; Thomas J Park Journal: J Comp Physiol A Neuroethol Sens Neural Behav Physiol Date: 2005-01-13 Impact factor: 1.836
Authors: Paolo Codega; Luca Della Santina; Claudia Gargini; Diana E Bedolla; Tatiana Subkhankulova; Frederick J Livesey; Luigi Cervetto; Vincent Torre Journal: J Physiol Date: 2009-03-30 Impact factor: 5.182
Authors: Lawrence H Pinto; Martha Hotz Vitaterna; Sanda M Siepka; Kazuhiro Shimomura; Stephen Lumayag; Matthew Baker; Deborah Fenner; Robert F Mullins; Val C Sheffield; Edwin M Stone; Edward Heffron; Joseph S Takahashi Journal: Vision Res Date: 2004-12 Impact factor: 1.886