Literature DB >> 12734261

Multiplex PCR for detection of three plasmid-borne genes of enteroaggregative Escherichia coli strains.

Jorge F Cerna1, James P Nataro, Teresa Estrada-Garcia.   

Abstract

We developed a novel multiplex PCR assay for enteroaggregative Escherichia coli (EAEC) detection, by using three plasmid-borne genes (the aggregative adherence [AA] probe, aap, and aggR). One or more of the loci were detected in 24 (86%) of 28 patient isolates analyzed. The multiplex PCR assay is a fast, convenient, and sensitive molecular test to detect EAEC.

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Year:  2003        PMID： 12734261      PMCID： PMC154749          DOI： 10.1128/JCM.41.5.2138-2140.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  22 in total

1.  A sensitive and specific DNA probe to identify enteroaggregative Escherichia coli, a recently discovered diarrheal pathogen.

Authors:  B Baudry; S J Savarino; P Vial; J B Kaper; M M Levine
Journal:  J Infect Dis       Date:  1990-06       Impact factor: 5.226

2.  Pathogenicity of enteroadherent Escherichia coli in adult volunteers.

Authors:  J J Mathewson; P C Johnson; H L DuPont; T K Satterwhite; D K Winsor
Journal:  J Infect Dis       Date:  1986-09       Impact factor: 5.226

3.  Association of Escherichia coli HEp-2 adherence patterns with type and duration of diarrhoea.

Authors:  A Cravioto; A Tello; A Navarro; J Ruiz; H Villafán; F Uribe; C Eslava
Journal:  Lancet       Date:  1991-02-02       Impact factor: 79.321

4.  Heterogeneity of enteroaggregative Escherichia coli virulence demonstrated in volunteers.

Authors:  J P Nataro; Y Deng; S Cookson; A Cravioto; S J Savarino; L D Guers; M M Levine; C O Tacket
Journal:  J Infect Dis       Date:  1995-02       Impact factor: 5.226

5.  Aggregative adherence fimbria I expression in enteroaggregative Escherichia coli requires two unlinked plasmid regions.

Authors:  J P Nataro; D Yikang; J A Giron; S J Savarino; M H Kothary; R Hall
Journal:  Infect Immun       Date:  1993-03       Impact factor: 3.441

6.  Enteroaggregative Escherichia coli and Salmonella associated with nondysenteric persistent diarrhea.

Authors:  M K Bhan; V Khoshoo; H Sommerfelt; P Raj; S Sazawal; R Srivastava
Journal:  Pediatr Infect Dis J       Date:  1989-08       Impact factor: 2.129

7.  Development of PCR for screening of enteroaggregative Escherichia coli.

Authors:  H Schmidt; C Knop; S Franke; S Aleksic; J Heesemann; H Karch
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948

8.  Aggregative adherence fimbriae I of enteroaggregative Escherichia coli mediate adherence to HEp-2 cells and hemagglutination of human erythrocytes.

Authors:  J P Nataro; Y Deng; D R Maneval; A L German; W C Martin; M M Levine
Journal:  Infect Immun       Date:  1992-06       Impact factor: 3.441

9.  AggR, a transcriptional activator of aggregative adherence fimbria I expression in enteroaggregative Escherichia coli.

Authors:  J P Nataro; D Yikang; D Yingkang; K Walker
Journal:  J Bacteriol       Date:  1994-08       Impact factor: 3.490

10.  Single multiplex polymerase chain reaction to detect diverse loci associated with diarrheagenic Escherichia coli.

Authors:  Catalina López-Saucedo; Jorge F Cerna; Nicolas Villegas-Sepulveda; Rocío Thompson; F Raul Velazquez; Javier Torres; Phillip I Tarr; Teresa Estrada-García
Journal:  Emerg Infect Dis       Date:  2003-01       Impact factor: 6.883
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  51 in total

1.  Genetic characterization of Escherichia coli O104 isolates from different sources in the United States.

Authors:  Lydia V Rump; Sonya Bodeis-Jones; Jason Abbott; Shaohua Zhao; Julie Kase; Sandra Lorenz; Markus Fischer; Eric Brown; Jianghong Meng
Journal:  Appl Environ Microbiol       Date:  2011-12-30       Impact factor: 4.792

2.  Association of putative pathogenicity genes with adherence characteristics and fimbrial genotypes in typical enteroaggregative Escherichia coli from patients with and without diarrhoea in the United Kingdom.

Authors:  C Jenkins; H Chart; G A Willshaw; T Cheasty; D S Tompkins
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2007-12       Impact factor: 3.267

3.  Evaluation of a multiplex PCR for identification of enteroaggregative Escherichia coli.

Authors:  Fabiana Cordeiro; Denise da Silva Gomes Pereira; Myrna Rocha; Marise Dutra Asensi; Waldir Pereira Elias; Leila Carvalho Campos
Journal:  J Clin Microbiol       Date:  2007-12-12       Impact factor: 5.948

4.  Prevalences of Shiga toxin subtypes and selected other virulence factors among Shiga-toxigenic Escherichia coli strains isolated from fresh produce.

Authors:  Peter C H Feng; Shanker Reddy
Journal:  Appl Environ Microbiol       Date:  2013-08-30       Impact factor: 4.792

5.  Origin and Evolution of Hybrid Shiga Toxin-Producing and Uropathogenic Escherichia coli Strains of Sequence Type 141.

Authors:  Noble Selasi Gati; Barbara Middendorf-Bauchart; Stefan Bletz; Ulrich Dobrindt; Alexander Mellmann
Journal:  J Clin Microbiol       Date:  2019-12-23       Impact factor: 5.948

6.  Enteroaggregative Escherichia coli infection induces IL-8 production via activation of mitogen-activated protein kinases and the transcription factors NF-kappaB and AP-1 in INT-407 cells.

Authors:  Krishnendu Khan; Monica Konar; Atul Goyal; Sujata Ghosh
Journal:  Mol Cell Biochem       Date:  2009-10-24       Impact factor: 3.396

7.  Narrowing of the Diagnostic Gap of Acute Gastroenteritis in Children 0-6 Years of Age Using a Combination of Classical and Molecular Techniques, Delivers Challenges in Syndromic Approach Diagnostics.

Authors:  Andrej Steyer; Monika Jevšnik; Miroslav Petrovec; Marko Pokorn; Štefan Grosek; Adela Fratnik Steyer; Barbara Šoba; Tina Uršič; Tjaša Cerar Kišek; Marko Kolenc; Marija Trkov; Petra Šparl; Raja Duraisamy; W Ian Lipkin; Sara Terzić; Mojca Kolnik; Tatjana Mrvič; Amit Kapoor; Franc Strle
Journal:  Pediatr Infect Dis J       Date:  2016-09       Impact factor: 2.129

Review 8.  Recent advances in understanding enteric pathogenic Escherichia coli.

Authors:  Matthew A Croxen; Robyn J Law; Roland Scholz; Kristie M Keeney; Marta Wlodarska; B Brett Finlay
Journal:  Clin Microbiol Rev       Date:  2013-10       Impact factor: 26.132

9.  Prevalence of enteroaggregative Escherichia coli and its virulence-related genes in a case-control study among children from north-eastern Brazil.

Authors:  Ila Fernanda Nunes Lima; Nadia Boisen; Josiane da Quetz Silva; Alexandre Havt; Eunice Bobo de Carvalho; Alberto Melo Soares; Noélia Leal Lima; Rosa Maria Salani Mota; James P Nataro; Richard Littleton Guerrant; Aldo Ângelo Moreira Lima
Journal:  J Med Microbiol       Date:  2013-02-21       Impact factor: 2.472

10.  Enteroaggregative Escherichia coli related to uropathogenic clonal group A.

Authors:  Faith Wallace-Gadsden; James R Johnson; John Wain; Iruka N Okeke
Journal:  Emerg Infect Dis       Date:  2007-05       Impact factor: 6.883
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