Literature DB >> 12730187

The yggH gene of Escherichia coli encodes a tRNA (m7G46) methyltransferase.

Lara G S De Bie1, Martine Roovers, Yamina Oudjama, Ruddy Wattiez, Catherine Tricot, Victor Stalon, Louis Droogmans, Janusz M Bujnicki.   

Abstract

We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-L-methionine-dependent formation of N(7)-methylguanosine at position 46 (m(7)G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant strain lacks tRNA (m(7)G46) methyltransferase activity.

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Year:  2003        PMID: 12730187      PMCID: PMC154064          DOI: 10.1128/JB.185.10.3238-3243.2003

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  15 in total

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9.  Structural basis for the methylation of G1405 in 16S rRNA by aminoglycoside resistance methyltransferase Sgm from an antibiotic producer: a diversity of active sites in m7G methyltransferases.

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