Literature DB >> 1271979

Quantitative determination of organic solvent soluble lipofuscin pigments in tissues.

A S Csallany, K L Ayaz.   

Abstract

Lipofuscin pigment determination in tissue extracts was quantitated by the use of its property of fluorescence. Chloroform:methanol tissue extracts were purified on Sephadex LH-20 columns before quantitative fluorescence measurements of the lipofuscin pigments. Interfering compounds separated by chromatography were retinol and a lower mol wt fluorescent compound. Irradiation of tissue extracts with ultraviolet light was not sufficient to eliminate the interference caused by retional and the lower mol wt compound. Purified lipofuscin pigments from blood, lung, liver, spleen, brain, heart, and kidney tissues demonstrated distinct fluorescent emission maximum at 435 nm and excitation maxima between 345 and 350 nm.

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Year:  1976        PMID： 1271979     DOI： 10.1007/BF02532849

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  10 in total

1.  Fractionation and analysis of fluorescent products of lipid peroxidation.

Authors:  R Trombly; A Tappel
Journal:  Lipids       Date:  1975-08       Impact factor: 1.880

2.  Studies on chemical nature of lipofusion (age pigment) isolated from normal human brain.

Authors:  R D Taubold
Journal:  Lipids       Date:  1975-07       Impact factor: 1.880

3.  Procedures for the isolation of two distinct lipopigments from human brain: lipofuscin and ceroid.

Authors:  A N Siakotos; I Watanabe; A Saito; S Fleischer
Journal:  Biochem Med       Date:  1970-12

4.  Measurement of fluorescent lipid peroxidation products in biological systems and tissues.

Authors:  B L Fletcher; C J Dillard; A L Tappel
Journal:  Anal Biochem       Date:  1973-03       Impact factor: 3.365

5.  Measurement and spectral characteristics of fluorescent pigments in tissues of rats as a function of dietary polyunsaturated fats and vitamin E.

Authors:  K Reddy; B Fletcher; A Tappel; A Tappel
Journal:  J Nutr       Date:  1973-06       Impact factor: 4.798

6.  Fluorescent products of lipid peroxidation of mitochondria and microsomes.

Authors:  C J Dillard; A L Tappel
Journal:  Lipids       Date:  1971-10       Impact factor: 1.880

7.  Synthesis and characterization of the fluorescent products derived from malonaldehyde and amino acids.

Authors:  K S Chio; A L Tappel
Journal:  Biochemistry       Date:  1969-07       Impact factor: 3.162

8.  Inactivation of ribonuclease and other enzymes by peroxidizing lipids and by malonaldehyde.

Authors:  K S Chio; A L Tappel
Journal:  Biochemistry       Date:  1969-07       Impact factor: 3.162

9.  Fluorescent products from aging Drosophila melanogaster: an indicator of free radical lipid peroxidation damage.

Authors:  J A Sheldahl; A L Tappel
Journal:  Exp Gerontol       Date:  1974-02       Impact factor: 4.032

10.  Peroxidation of subcellular organelles: formation of lipofuscinlike fluorescent pigments.

Authors:  K S Chio; U Reiss; B Fletcher; A L Tappel
Journal:  Science       Date:  1969-12-19       Impact factor: 47.728
  10 in total
  14 in total

1.  HPLC method for quantitation of cholesterol and four of its major oxidation products in muscle and liver tissues.

Authors:  A S Csallany; S E Kindom; P B Addis; J H Lee
Journal:  Lipids       Date:  1989-07       Impact factor: 1.880

2.  Time-course of changes in lipofuscin-like pigments in rat liver homogenate and mitochondria after whole body gamma irradiation.

Authors:  J Wilhelm; J Sonka
Journal:  Experientia       Date:  1981-06

3.  Blood lipid profile and myocardial superoxide dismutase in swim-trained young and middle-aged rats: comparison between left and right ventricular adaptations to oxidative stress.

Authors:  T Ravi Kiran; M V V Subramanyam; S Prathima; S Asha Devi
Journal:  J Comp Physiol B       Date:  2006-06-20       Impact factor: 2.200

4.  Fluorescent substances derived from the reaction of 13-monohydroperoxylinoleic acid and methylamine.

Authors:  K Kikugawa; S Watanabe
Journal:  Lipids       Date:  1988-04       Impact factor: 1.880

5.  Isolation and analysis of age-related fluorescent substances in rat testes.

Authors:  H Shimasaki; N Ueta; O S Privett
Journal:  Lipids       Date:  1980-04       Impact factor: 1.880

6.  Malondialdehyde-containing proteins and their relationship to vitamin E.

Authors:  J D Manwaring; A S Csallany
Journal:  Lipids       Date:  1988-07       Impact factor: 1.880

7.  Lipofuscin in vitamin E deficiency and the possible role of retinol.

Authors:  J G Bieri; T J Tolliver; W G Robison; T Kuwabara
Journal:  Lipids       Date:  1980-01       Impact factor: 1.880

Review 8.  Disturbances of metabolism and cardiac function under the action of emotional painful stress and their prophylaxis.

Authors:  F Z Meerson
Journal:  Basic Res Cardiol       Date:  1980 Jul-Aug       Impact factor: 17.165

9.  Lipid fluorophores of the human crystalline lens with cataract.

Authors:  M A Babizhayev
Journal:  Graefes Arch Clin Exp Ophthalmol       Date:  1989       Impact factor: 3.117

10.  Fluorescent pigments by covalent binding of lipid peroxidation by-products to protein and amino acids.

Authors:  K Fukuzawa; K Kishikawa; A Tokumura; H Tsukatani; M Shibuya
Journal:  Lipids       Date:  1985-12       Impact factor: 1.880
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