Literature DB >> 12718929

A deliberate approach to screening for initial crystallization conditions of biological macromolecules.

Joseph R Luft1, Robert J Collins, Nancy A Fehrman, Angela M Lauricella, Christina K Veatch, George T DeTitta.   

Abstract

A method to rationally predict crystallization conditions for a previously uncrystallized macromolecule has not yet been developed. One way around this problem is to determine initial crystallization conditions by casting a wide net, surveying a large number of chemical and physical conditions to locate crystallization leads. A facility that executes the rapid survey of crystallization lead conditions is described in detail. Results and guidelines for the initial screening of crystallization conditions, applicable to both manual and robotic setups, are discussed.

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Year:  2003        PMID： 12718929     DOI： 10.1016/s1047-8477(03)00048-0

Source DB:  PubMed          Journal:  J Struct Biol        ISSN： 1047-8477            Impact factor:   2.867


  147 in total

1.  Laboratory scale structural genomics.

Authors:  Brent W Segelke; Johana Schafer; Matthew A Coleman; Tim P Lekin; Dominique Toppani; Krzysztof J Skowronek; Katherine A Kantardjieff; Bernhard Rupp
Journal:  J Struct Funct Genomics       Date:  2004

Review 2.  Protein crystallization in the structural genomics era.

Authors:  Alexander McPherson
Journal:  J Struct Funct Genomics       Date:  2004

3.  Crystallization and preliminary X-ray crystallographic studies of the outer membrane cytochrome OmcA from Shewanella oneidensis MR-1.

Authors:  S J Tomanicek; A Johs; M S Sawhney; L Shi; L Liang
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-12-24

4.  Trapping and structure determination of an intermediate in the allosteric transition of aspartate transcarbamoylase.

Authors:  Wenyue Guo; Jay M West; Andrew S Dutton; Hiro Tsuruta; Evan R Kantrowitz
Journal:  Proc Natl Acad Sci U S A       Date:  2012-04-30       Impact factor: 11.205

5.  The structure of vimentin linker 1 and rod 1B domains characterized by site-directed spin-labeling electron paramagnetic resonance (SDSL-EPR) and X-ray crystallography.

Authors:  Atya Aziz; John F Hess; Madhu S Budamagunta; John C Voss; Alexandre P Kuzin; Yuanpeng J Huang; Rong Xiao; Gaetano T Montelione; Paul G FitzGerald; John F Hunt
Journal:  J Biol Chem       Date:  2012-06-26       Impact factor: 5.157

6.  Structure determination of glycogen synthase kinase-3 from Leishmania major and comparative inhibitor structure-activity relationships with Trypanosoma brucei GSK-3.

Authors:  Kayode K Ojo; Tracy L Arakaki; Alberto J Napuli; Krishna K Inampudi; Katelyn R Keyloun; Li Zhang; Wim G J Hol; Christophe L M J Verlinde; Ethan A Merritt; Wesley C Van Voorhis
Journal:  Mol Biochem Parasitol       Date:  2010-12-30       Impact factor: 1.759

7.  Structure and function of the Escherichia coli protein YmgB: a protein critical for biofilm formation and acid-resistance.

Authors:  Jintae Lee; Rebecca Page; Rodolfo García-Contreras; Jeanne-Marie Palermino; Xue-Song Zhang; Ojus Doshi; Thomas K Wood; Wolfgang Peti
Journal:  J Mol Biol       Date:  2007-08-02       Impact factor: 5.469

8.  Structural characterization of the hemophore HasAp from Pseudomonas aeruginosa: NMR spectroscopy reveals protein-protein interactions between Holo-HasAp and hemoglobin.

Authors:  Aileen Y Alontaga; Juan Carlos Rodriguez; Ernst Schönbrunn; Andreas Becker; Todd Funke; Erik T Yukl; Takahiro Hayashi; Jordan Stobaugh; Pierre Moënne-Loccoz; Mario Rivera
Journal:  Biochemistry       Date:  2009-01-13       Impact factor: 3.162

9.  Human Glycerol 3-Phosphate Dehydrogenase: X-ray Crystal Structures That Guide the Interpretation of Mutagenesis Studies.

Authors:  Lisa S Mydy; Judith R Cristobal; Roberto D Katigbak; Paul Bauer; Archie C Reyes; Shina Caroline Lynn Kamerlin; John P Richard; Andrew M Gulick
Journal:  Biochemistry       Date:  2019-01-31       Impact factor: 3.162

10.  Purification, crystallization and preliminary X-ray diffraction analysis of pathogen-inducible oxygenase (PIOX) from Oryza sativa.

Authors:  Tracy Lloyd; Adam Krol; Danielle Campanaro; Michael Malkowski
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-03-10
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