Literature DB >> 12692249

Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons.

Rainer Gosert1, Denise Egger, Volker Lohmann, Ralf Bartenschlager, Hubert E Blum, Kurt Bienz, Darius Moradpour.   

Abstract

Formation of a membrane-associated replication complex, composed of viral proteins, replicating RNA, and altered cellular membranes, is a characteristic feature of plus-strand RNA viruses. Here, we demonstrate the presence of a specific membrane alteration, designated the membranous web, that contains hepatitis C virus (HCV) nonstructural proteins, as well as viral plus-strand RNA, in Huh-7 cells harboring autonomously replicating subgenomic HCV RNAs. Metabolic labeling with 5-bromouridine 5'-triphosphate in the presence of actinomycin D revealed that the membranous web is the site of viral RNA synthesis and therefore represents the replication complex of HCV.

Entities:  

Mesh：

Substances：

Year:  2003        PMID： 12692249      PMCID： PMC153965          DOI： 10.1128/jvi.77.9.5487-5492.2003

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  32 in total

1.  The hepatitis C virus nonstructural protein 4B is an integral endoplasmic reticulum membrane protein.

Authors:  T Hügle; F Fehrmann; E Bieck; M Kohara; H G Kräusslich; C M Rice; H E Blum; D Moradpour
Journal:  Virology       Date:  2001-05-25       Impact factor: 3.616

2.  A cytopathic and a cell culture adapted hepatitis A virus strain differ in cell killing but not in intracellular membrane rearrangements.

Authors:  R Gosert; D Egger; K Bienz
Journal:  Virology       Date:  2000-01-05       Impact factor: 3.616

3.  Semliki Forest virus mRNA capping enzyme requires association with anionic membrane phospholipids for activity.

Authors:  T Ahola; A Lampio; P Auvinen; L Kääriäinen
Journal:  EMBO J       Date:  1999-06-01       Impact factor: 11.598

4.  Non-structural proteins 2 and 3 interact to modify host cell membranes during the formation of the arterivirus replication complex.

Authors:  Eric J Snijder; Hans van Tol; Norbert Roos; Ketil W Pedersen
Journal:  J Gen Virol       Date:  2001-05       Impact factor: 3.891

5.  Requirements for assembly of poliovirus replication complexes and negative-strand RNA synthesis.

Authors:  N L Teterina; D Egger; K Bienz; D M Brown; B L Semler; E Ehrenfeld
Journal:  J Virol       Date:  2001-04       Impact factor: 5.103

6.  Selectable subgenomic and genome-length dicistronic RNAs derived from an infectious molecular clone of the HCV-N strain of hepatitis C virus replicate efficiently in cultured Huh7 cells.

Authors:  Masanori Ikeda; MinKyung Yi; Kui Li; Stanley M Lemon
Journal:  J Virol       Date:  2002-03       Impact factor: 5.103

7.  Mutations in hepatitis C virus RNAs conferring cell culture adaptation.

Authors:  V Lohmann; F Körner; A Dobierzewska; R Bartenschlager
Journal:  J Virol       Date:  2001-02       Impact factor: 5.103

8.  Characterization of cell lines carrying self-replicating hepatitis C virus RNAs.

Authors:  T Pietschmann; V Lohmann; G Rutter; K Kurpanek; R Bartenschlager
Journal:  J Virol       Date:  2001-02       Impact factor: 5.103

9.  Efficient initiation of HCV RNA replication in cell culture.

Authors:  K J Blight; A A Kolykhalov; C M Rice
Journal:  Science       Date:  2000-12-08       Impact factor: 47.728

10.  Determinants for membrane association of the hepatitis C virus RNA-dependent RNA polymerase.

Authors:  J Schmidt-Mende; E Bieck; T Hugle; F Penin; C M Rice; H E Blum; D Moradpour
Journal:  J Biol Chem       Date:  2001-11-23       Impact factor: 5.157
View more
  301 in total

1.  Amphipathic alpha-helix AH2 is a major determinant for the oligomerization of hepatitis C virus nonstructural protein 4B.

Authors:  Jérôme Gouttenoire; Philippe Roingeard; François Penin; Darius Moradpour
Journal:  J Virol       Date:  2010-10-06       Impact factor: 5.103

2.  In-cell click labelling of small molecules to determine subcellular localisation.

Authors:  Lyn H Jones; David Beal; Matthew D Selby; Owen Everson; George M Burslem; Peter Dodd; Jared Millbank; Thien-Duc Tran; Florian Wakenhut; Emily J S Graham; Paul Targett-Adams
Journal:  J Chem Biol       Date:  2010-11-18

Review 3.  Hepatitis C virus: assembly and release of virus particles.

Authors:  Daniel M Jones; John McLauchlan
Journal:  J Biol Chem       Date:  2010-05-10       Impact factor: 5.157

4.  A genetic interaction between the core and NS3 proteins of hepatitis C virus is essential for production of infectious virus.

Authors:  Daniel M Jones; Ali M Atoom; Xiaozhen Zhang; Shyamasundaran Kottilil; Rodney S Russell
Journal:  J Virol       Date:  2011-09-28       Impact factor: 5.103

Review 5.  A guide to viral inclusions, membrane rearrangements, factories, and viroplasm produced during virus replication.

Authors:  Christopher Netherton; Katy Moffat; Elizabeth Brooks; Thomas Wileman
Journal:  Adv Virus Res       Date:  2007       Impact factor: 9.937

6.  Hepatitis C virus core protein activates autophagy through EIF2AK3 and ATF6 UPR pathway-mediated MAP1LC3B and ATG12 expression.

Authors:  Ji Wang; Rongyan Kang; He Huang; Xueyan Xi; Bei Wang; Jianwei Wang; Zhendong Zhao
Journal:  Autophagy       Date:  2014-02-20       Impact factor: 16.016

7.  Responses of nontransformed human hepatocytes to conditional expression of full-length hepatitis C virus open reading frame.

Authors:  Weiliang Tang; Catherine A Lázaro; Jean S Campbell; W Tony Parks; Michael G Katze; Nelson Fausto
Journal:  Am J Pathol       Date:  2007-11-08       Impact factor: 4.307

8.  Analysis of hepatitis C virus resistance to silibinin in vitro and in vivo points to a novel mechanism involving nonstructural protein 4B.

Authors:  Katharina Esser-Nobis; Inés Romero-Brey; Tom M Ganten; Jérôme Gouttenoire; Christian Harak; Rahel Klein; Peter Schemmer; Marco Binder; Paul Schnitzler; Darius Moradpour; Ralf Bartenschlager; Stephen J Polyak; Wolfgang Stremmel; François Penin; Christoph Eisenbach; Volker Lohmann
Journal:  Hepatology       Date:  2013-02-07       Impact factor: 17.425

9.  The hepatitis C virus NS4B protein can trans-complement viral RNA replication and modulates production of infectious virus.

Authors:  Daniel M Jones; Arvind H Patel; Paul Targett-Adams; John McLauchlan
Journal:  J Virol       Date:  2008-12-10       Impact factor: 5.103

10.  Hepatitis C virus-host interactions: Etiopathogenesis and therapeutic strategies.

Authors:  Mohamed Hassan; Denis Selimovic; Abdelouahid El-Khattouti; Hanan Ghozlan; Youssef Haikel; Ola Abdelkader
Journal:  World J Exp Med       Date:  2012-04-20
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.