| Literature DB >> 12670980 |
Jeremy W Dale1, Hasan Al-Ghusein, Salim Al-Hashmi, Philip Butcher, Anne L Dickens, Francis Drobniewski, Ken J Forbes, Stephen H Gillespie, Dianie Lamprecht, Timothy D McHugh, Richard Pitman, Nalin Rastogi, Andrew T Smith, Christophe Sola, Hasan Yesilkaya.
Abstract
Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a study of the epidemiology of tuberculosis in London, we have shown a substantial degree of congruence between IS6110 patterns and both spoligotype and PGRS type. This indicates that the IS6110 types mainly represent distinct families of strains rather than arising through the convergent insertion of IS6110 into favored positions. This is supported by identification of the genomic sites of the insertion of IS6110 in these strains. The combined data enable identification of the putative evolutionary relationships of these strains, comprising three lineages broadly associated with patients born in South Asia (India and Pakistan), Africa, and Europe, respectively. These lineages appear to be quite distinct from M. tuberculosis isolates with multiple copies of IS6110.Entities:
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Year: 2003 PMID： 12670980 PMCID： PMC152614 DOI： 10.1128/JB.185.8.2555-2562.2003
Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490