Literature DB >> 12661662

Technical note: Specific PCR amplification of protozoal 18S rDNA sequences from DNA extracted from ruminal samples of cows.

S K R Karnati1, Z Yu, J T Sylvester, B A Dehority, M Morrison, J L Firkins.   

Abstract

A protozoa-specific primer (P-SSU-342f) was designed and paired with a eukarya-specific primer to amplify a 1,360-bp fragment of DNA encoding protozoal small subunit (SSU) ribosomal RNA from ruminal fluid of cows fed a mixed forage:grain diet or alfalfa hay. Sequencing of clones showed that P-SSU-342f is specific to ruminal protozoa and, with slight modifications, the primer will be useful for ecological studies of ruminal protozoa.

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Year:  2003        PMID： 12661662     DOI： 10.2527/2003.813812x

Source DB:  PubMed          Journal:  J Anim Sci        ISSN： 0021-8812            Impact factor:   3.159


  14 in total

1.  New Primers Targeting Full-Length Ciliate 18S rRNA Genes and Evaluation of Dietary Effect on Rumen Ciliate Diversity in Dairy Cows.

Authors:  Jun Zhang; Shengguo Zhao; Yangdong Zhang; Peng Sun; Dengpan Bu; Jiaqi Wang
Journal:  Curr Microbiol       Date:  2015-08-30       Impact factor: 2.188

2.  Development and validation of a real-time PCR method to quantify rumen protozoa and examination of variability between entodinium populations in sheep offered a hay-based diet.

Authors:  Lucy C Skillman; Andrew F Toovey; Andrew J Williams; André-Denis G Wright
Journal:  Appl Environ Microbiol       Date:  2006-01       Impact factor: 4.792

3.  Postinoculation protozoan establishment and association patterns of methanogenic archaea in the ovine rumen.

Authors:  Samuel Ohene-Adjei; Ronald M Teather; Michael Ivan; Robert J Forster
Journal:  Appl Environ Microbiol       Date:  2007-05-18       Impact factor: 4.792

Review 4.  The use of molecular techniques based on ribosomal RNA and DNA for rumen microbial ecosystem studies: a review.

Authors:  Weidong Deng; Dongmei Xi; Huaming Mao; Metha Wanapat
Journal:  Mol Biol Rep       Date:  2007-05-05       Impact factor: 2.316

5.  An efficient RNA extraction method for estimating gut microbial diversity by polymerase chain reaction.

Authors:  Seungha Kang; Stuart E Denman; Mark Morrison; Zhongtang Yu; Chris S McSweeney
Journal:  Curr Microbiol       Date:  2009-01-22       Impact factor: 2.188

6.  Eukaryotic Community Shift in Response to Organic Loading Rate of an Aerobic Trickling Filter (Down-Flow Hanging Sponge Reactor) Treating Domestic Sewage.

Authors:  Yuma Miyaoka; Masashi Hatamoto; Takashi Yamaguchi; Kazuaki Syutsubo
Journal:  Microb Ecol       Date:  2016-10-28       Impact factor: 4.552

7.  Design and validation of four new primers for next-generation sequencing to target the 18S rRNA genes of gastrointestinal ciliate protozoa.

Authors:  Suzanne L Ishaq; André-Denis G Wright
Journal:  Appl Environ Microbiol       Date:  2014-06-27       Impact factor: 4.792

8.  Dasytricha dominance in Surti buffalo rumen revealed by 18S rRNA sequences and real-time PCR assay.

Authors:  K M Singh; A K Tripathi; P R Pandya; D N Rank; R K Kothari; C G Joshi
Journal:  Curr Microbiol       Date:  2011-07-10       Impact factor: 2.188

9.  Prevalence of protozoa species in drinking and environmental water sources in Sudan.

Authors:  Salah Shanan; Hadi Abd; Magdi Bayoumi; Amir Saeed; Gunnar Sandström
Journal:  Biomed Res Int       Date:  2015-02-18       Impact factor: 3.411

10.  AmpliconDuo: A Split-Sample Filtering Protocol for High-Throughput Amplicon Sequencing of Microbial Communities.

Authors:  Anja Lange; Steffen Jost; Dominik Heider; Christina Bock; Bettina Budeus; Elmar Schilling; Axel Strittmatter; Jens Boenigk; Daniel Hoffmann
Journal:  PLoS One       Date:  2015-11-02       Impact factor: 3.240
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