BACKGROUND: Intratracheal injection of manganese superoxide dismutase-plasmid/liposome (MnSOD-PL) prior to single fraction or fractionated irradiation of C57BL/6J mouse lung has been demonstrated to protect the lung from irradiation-induced damage. MATERIALS AND METHODS: To determine whether irradiation-induced inflammatory cytokine levels influenced the recovery of tumors following single fraction irradiation, mice with orthotopic Lewis Lung Carcinoma (3LL) tumors received MnSOD-PL treatment 24 hours after tumor implantation and 24 hours prior to irradiation. Subgroups were implanted with Alzet pumps continuously replacing levels of inflammatory cytokines over 7 days. RESULTS: In cytokine-treated mice, there was no detectable significant alteration in radiotherapy-mediated improved survival (tumor regrowth delay) compared to irradiated control mice. Each group of mice that received MnSOD-PL had increased survival compared to irradiated controls. CONCLUSION: These results support the anticipated safety of intrapulmonary MnSOD-PL gene therapy in lung cancer patients for protection of normal lung tissue from irradiation while allowing effective irradiation-mediated tumor control.
BACKGROUND: Intratracheal injection of manganese superoxide dismutase-plasmid/liposome (MnSOD-PL) prior to single fraction or fractionated irradiation of C57BL/6J mouse lung has been demonstrated to protect the lung from irradiation-induced damage. MATERIALS AND METHODS: To determine whether irradiation-induced inflammatory cytokine levels influenced the recovery of tumors following single fraction irradiation, mice with orthotopic Lewis Lung Carcinoma (3LL) tumors received MnSOD-PL treatment 24 hours after tumor implantation and 24 hours prior to irradiation. Subgroups were implanted with Alzet pumps continuously replacing levels of inflammatory cytokines over 7 days. RESULTS: In cytokine-treated mice, there was no detectable significant alteration in radiotherapy-mediated improved survival (tumor regrowth delay) compared to irradiated control mice. Each group of mice that received MnSOD-PL had increased survival compared to irradiated controls. CONCLUSION: These results support the anticipated safety of intrapulmonary MnSOD-PL gene therapy in lung cancerpatients for protection of normal lung tissue from irradiation while allowing effective irradiation-mediated tumor control.
Authors: Molly S Stitt-Fischer; Rachel K Ungerman; Daniel S Wilen; Karla Wasserloos; Lara M Renz; Shannon E Raub; Jim Peterson; Linda L Pearce Journal: Radiat Res Date: 2010-09-28 Impact factor: 2.841
Authors: Michael W Epperly; Julie P Goff; Song Li; Xiang Gao; Peter Wipf; Tracy Dixon; Hong Wang; Darcy Franicola; Hongmei Shen; Jean-Claude M Rwigema; Valerian Kagan; Mark Bernard; Joel S Greenberger Journal: In Vivo Date: 2010 Nov-Dec Impact factor: 2.155
Authors: Wenqing G Sun; Christine J Weydert; Yuping Zhang; Lei Yu; Jingru Liu; Douglas R Spitz; Joseph J Cullen; Larry W Oberley Journal: Cancers (Basel) Date: 2010-02-12 Impact factor: 6.639
Authors: Marlon R Veldwijk; Carsten Herskind; Leopold Sellner; Aleksandar Radujkovic; Stephanie Laufs; Stefan Fruehauf; W Jens Zeller; Frederik Wenz Journal: Strahlenther Onkol Date: 2009-08-04 Impact factor: 3.621
Authors: Pataje G S Prasanna; Helen B Stone; Rosemary S Wong; Jacek Capala; Eric J Bernhard; Bhadrasain Vikram; C N Coleman Journal: Transl Cancer Res Date: 2012-06 Impact factor: 1.241