BACKGROUND/AIM: Use of zebrafish models may decrease the cost of screening new irradiation protectors and mitigators. MATERIALS AND METHODS: Zebrafish (Danio rerio) models were tested for screening water-soluble radiation protectors and mitigators. Irradiation of embryos and monitoring survival, and measuring fibrosis of the caudal musculature of adults allowed for testing of acute and late effects, respectively. RESULTS: Incubation of zebrafish embryos either before or after irradiation in ethyl pyruvate (1 mM) increased survival. Irradiation of adults to 15 to 75 Gy, delivered in single-fraction at 13 Gy/min, showed dose-dependent fibrosis at 30 days, quantitated as physiological decrease in swimming tail movement, and histopathological detection of collagen deposition in the dorsal musculature. Continuous administration of small-molecule radioprotector drugs in the water after irradiation reduced both acute and chronic injuries. CONCLUSION: The zebrafish is cost-effective for screening new radiation countermeasures.
BACKGROUND/AIM: Use of zebrafish models may decrease the cost of screening new irradiation protectors and mitigators. MATERIALS AND METHODS:Zebrafish (Danio rerio) models were tested for screening water-soluble radiation protectors and mitigators. Irradiation of embryos and monitoring survival, and measuring fibrosis of the caudal musculature of adults allowed for testing of acute and late effects, respectively. RESULTS: Incubation of zebrafish embryos either before or after irradiation in ethyl pyruvate (1 mM) increased survival. Irradiation of adults to 15 to 75 Gy, delivered in single-fraction at 13 Gy/min, showed dose-dependent fibrosis at 30 days, quantitated as physiological decrease in swimming tail movement, and histopathological detection of collagen deposition in the dorsal musculature. Continuous administration of small-molecule radioprotector drugs in the water after irradiation reduced both acute and chronic injuries. CONCLUSION: The zebrafish is cost-effective for screening new radiation countermeasures.
Authors: Jean-Claude M Rwigema; Barbara Beck; Wei Wang; Alexander Doemling; Michael W Epperly; Donna Shields; Julie P Goff; Darcy Franicola; Tracy Dixon; Marie-Céline Frantz; Peter Wipf; Yulia Tyurina; Valerian E Kagan; Hong Wang; Joel S Greenberger Journal: Int J Radiat Oncol Biol Phys Date: 2011-04-13 Impact factor: 7.038
Authors: Yaron Perry; Michael W Epperly; Hiran C Fernando; Ed Klein; Sid Finkelstein; Joel S Greenberger; James D Luketich Journal: J Surg Res Date: 2005-01 Impact factor: 2.192
Authors: Michael W Epperly; Darcy Franicola; Xichen Zhang; Suhua Nie; Hong Wang; Alfred B Bahnson; Donna S Shields; Julie P Goff; Hongmei Shen; Joel S Greenberger Journal: In Vivo Date: 2006 Sep-Oct Impact factor: 2.155
Authors: Michael W Epperly; Darcy Franicola; Donna Shields; Jean-Claude Rwigema; Brandon Stone; Xichen Zhang; William McBride; George Georges; Peter Wipf; Joel S Greenberger Journal: In Vivo Date: 2010 Jan-Feb Impact factor: 2.155
Authors: Michael W Epperly; Hongliang Guo; Hongmei Shen; Yunyun Niu; Xichen Zhang; Mia Jefferson; Christine A Sikora; Joel S Greenberger Journal: Radiat Res Date: 2004-09 Impact factor: 2.841
Authors: Jianfei Jiang; Peter R McDonald; Tracy M Dixon; Darcy Franicola; Xichen Zhang; Suhua Nie; Laura D Epperly; Zhentai Huang; Valerian E Kagan; John S Lazo; Michael W Epperly; Joel S Greenberger Journal: Radiat Res Date: 2009-10 Impact factor: 2.841
Authors: Malolan S Rajagopalan; Kanika Gupta; Michael W Epperly; Darcy Franicola; Xichen Zhang; Hong Wang; Hong Zhao; Vladimir A Tyurin; Joshua G Pierce; Valerian E Kagan; Peter Wipf; Anthony J Kanai; Joel S Greenberger Journal: In Vivo Date: 2009 Sep-Oct Impact factor: 2.155
Authors: J S Greenberger; M W Epperly; J Gretton; M Jefferson; S Nie; M Bernarding; V Kagan; H L Guo Journal: Curr Gene Ther Date: 2003-06 Impact factor: 4.391