PURPOSE: The effect of recombinant human erythropoietin (rhEPO) on the radiosensitivity of human tumor xenografts growing in anemic and nonanemic nude mice was studied. METHODS AND MATERIALS: Anemia was induced by total body irradiation ([TBI], 2 x 4 Gy) of mice before tumor implantation into the subcutis of the hind leg. The development of anemia was prevented by rhEPO (750 U/kg s.c.) given 3 times weekly starting 2 weeks before TBI. Fourteen days after fractionated TBI (tumor volume of approx. 40 mm(3)), single-dose irradiation of the tumor with varying doses was performed so that in full dose-response relationship for the probability of tumor cure was obtained. RESULTS: Radiation-induced anemia (hemoglobin concentration [cHb] = 9.9 g/dl) led to a reduced radiosensitivity compared to controls [49.4 vs. 40.1 Gy radiation dose to control 50% of the tumors (TCD50)]. Upon rhEPO treatment for anemia prevention (cHb = 13.3 g/dl), the TCD50 was 39.8 Gy, illustrating restored radiosensitivity compared to anemic mice. CONCLUSION: These data provide further experimental evidence for restored radiosensitivity upon prevention of anemia with rhEPO.
PURPOSE: The effect of recombinant humanerythropoietin (rhEPO) on the radiosensitivity of humantumor xenografts growing in anemic and nonanemic nude mice was studied. METHODS AND MATERIALS: Anemia was induced by total body irradiation ([TBI], 2 x 4 Gy) of mice before tumor implantation into the subcutis of the hind leg. The development of anemia was prevented by rhEPO (750 U/kg s.c.) given 3 times weekly starting 2 weeks before TBI. Fourteen days after fractionated TBI (tumor volume of approx. 40 mm(3)), single-dose irradiation of the tumor with varying doses was performed so that in full dose-response relationship for the probability of tumor cure was obtained. RESULTS: Radiation-induced anemia (hemoglobin concentration [cHb] = 9.9 g/dl) led to a reduced radiosensitivity compared to controls [49.4 vs. 40.1 Gy radiation dose to control 50% of the tumors (TCD50)]. Upon rhEPO treatment for anemia prevention (cHb = 13.3 g/dl), the TCD50 was 39.8 Gy, illustrating restored radiosensitivity compared to anemicmice. CONCLUSION: These data provide further experimental evidence for restored radiosensitivity upon prevention of anemia with rhEPO.
Authors: Huagang Hou; Ruhong Dong; Hongbin Li; Benjamin Williams; Jean P Lariviere; S K Hekmatyar; Risto A Kauppinen; Nadeem Khan; Harold Swartz Journal: J Magn Reson Date: 2011-10-01 Impact factor: 2.229
Authors: Huagang Hou; Ruhong Dong; Jean P Lariviere; Sriram P Mupparaju; Harold M Swartz; Nadeem Khan Journal: J Radiat Res Date: 2011-07-29 Impact factor: 2.724
Authors: W Ceelen; T Boterberg; P Smeets; N Van Damme; P Demetter; O Zwaenepoel; L Cesteleyn; P Houtmeyers; M Peeters; P Pattyn Journal: Br J Cancer Date: 2007-02-13 Impact factor: 7.640
Authors: Bernd Gagel; Patrick Reinartz; Cengiz Demirel; Hans J Kaiser; Michael Zimny; Marc Piroth; Michael Pinkawa; Sven Stanzel; Branka Asadpour; Kurt Hamacher; Heinz H Coenen; Ulrich Buell; Michael J Eble Journal: BMC Cancer Date: 2006-03-04 Impact factor: 4.430