Literature DB >> 12650530

Plastid transformation in Lesquerella fendleri, an oilseed Brassicacea.

Marina Skarjinskaia1, Zora Svab, Pal Maliga.   

Abstract

A plastid transformation protocol was developed for Lesquerella fendleri, a species with a high capacity for plant regeneration in tissue culture. Transformation vector pZS391B carried an aadA16gfp marker gene conferring streptomycin-spectinomycin resistance and green fluorescence under UV light. Biolistic transformation of 51 Lesquerella leaf samples, followed by spectinomycin selection, yielded two transplastomic clones. The AAD-GFP fusion protein, the marker gene product, was localized to chloroplasts by confocal laser microscopy. Fertile plants and seed progeny were obtained in line Lf-pZS391B-1. In the 51 samples a large number (108) of spontaneous mutants were identified. In five of the lines spectinomycin resistance was localized to a conserved stem structure by sequencing 16S rRNA genes. Success in L. fendleri, a wild oilseed species, extends plastid transformation beyond Arabidopsis thaliana in the Brassicaceae family.

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Year:  2003        PMID： 12650530     DOI： 10.1023/a:1022110402302

Source DB:  PubMed          Journal:  Transgenic Res        ISSN： 0962-8819            Impact factor:   2.788


  30 in total

Review 1.  Protein expression in plastids.

Authors:  P B Heifetz; A M Tuttle
Journal:  Curr Opin Plant Biol       Date:  2001-04       Impact factor: 7.834

Review 2.  Engineering the plastid genome of higher plants.

Authors:  Pal Maliga
Journal:  Curr Opin Plant Biol       Date:  2002-04       Impact factor: 7.834

Review 3.  Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology.

Authors:  Henry Daniell; Muhammad S Khan; Lori Allison
Journal:  Trends Plant Sci       Date:  2002-02       Impact factor: 18.313

4.  Efficient elimination of selectable marker genes from the plastid genome by the CRE-lox site-specific recombination system.

Authors:  S Corneille; K Lutz; Z Svab; P Maliga
Journal:  Plant J       Date:  2001-07       Impact factor: 6.417

5.  Stable transformation of plastids in higher plants.

Authors:  Z Svab; P Hajdukiewicz; P Maliga
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

Review 6.  The chloroplast genome.

Authors:  M Sugiura
Journal:  Plant Mol Biol       Date:  1992-05       Impact factor: 4.076

7.  Homeologous plastid DNA transformation in tobacco is mediated by multiple recombination events.

Authors:  T A Kavanagh; N D Thanh; N T Lao; N McGrath; S O Peter; E M Horváth; P J Dix; P Medgyesy
Journal:  Genetics       Date:  1999-07       Impact factor: 4.562

8.  Removal of antibiotic resistance genes from transgenic tobacco plastids.

Authors:  S Iamtham; A Day
Journal:  Nat Biotechnol       Date:  2000-11       Impact factor: 54.908

9.  Mutations conferring lincomycin, spectinomycin, and streptomycin resistance in Solanum nigrum are located in three different chloroplast genes.

Authors:  T A Kavanagh; K M O'Driscoll; P F McCabe; P J Dix
Journal:  Mol Gen Genet       Date:  1994-03

10.  Mutation proximal to the tRNA binding region of the Nicotiana plastid 16S rRNA confers resistance to spectinomycin.

Authors:  Z Svab; P Maliga
Journal:  Mol Gen Genet       Date:  1991-08
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  33 in total

1.  High-frequency transformation of undeveloped plastids in tobacco suspension cells.

Authors:  Camri L Langbecker; Guang-Ning Ye; Debra L Broyles; Lisa L Duggan; Charles W Xu; Peter T J Hajdukiewicz; Charles L Armstrong; Jeffrey M Staub
Journal:  Plant Physiol       Date:  2004-05       Impact factor: 8.340

2.  High efficiency plastid transformation in potato and regulation of transgene expression in leaves and tubers by alternative 5' and 3' regulatory sequences.

Authors:  Vladimir T Valkov; Daniela Gargano; Carmela Manna; Gelsomina Formisano; Philip J Dix; John C Gray; Nunzia Scotti; Teodoro Cardi
Journal:  Transgenic Res       Date:  2010-05-13       Impact factor: 2.788

3.  Construction of a species-specific vector for improved plastid transformation efficiency in Capsicum annuum L.

Authors:  Srinivas Kota; Raghuvardhan Lakkam; Kirnamayee Kasula; Muralikrishna Narra; Hao Qiang; V Rao Allini; Hu Zanmin; Sadanandam Abbagani
Journal:  3 Biotech       Date:  2019-05-21       Impact factor: 2.406

4.  Generation and analysis of soybean plastid transformants expressing Bacillus thuringiensis Cry1Ab protoxin.

Authors:  N Dufourmantel; G Tissot; F Goutorbe; F Garçon; C Muhr; S Jansens; B Pelissier; G Peltier; M Dubald
Journal:  Plant Mol Biol       Date:  2005-07       Impact factor: 4.076

Review 5.  Breakthrough in chloroplast genetic engineering of agronomically important crops.

Authors:  Henry Daniell; Shashi Kumar; Nathalie Dufourmantel
Journal:  Trends Biotechnol       Date:  2005-05       Impact factor: 19.536

6.  Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids.

Authors:  Hirosuke Kanamoto; Atsushi Yamashita; Hiroshi Asao; Satoru Okumura; Hisabumi Takase; Masahira Hattori; Akiho Yokota; Ken-Ichi Tomizawa
Journal:  Transgenic Res       Date:  2006-04       Impact factor: 2.788

7.  Genetic transformation of plastids of different Solanaceae species using tobacco cells as organelle hosts.

Authors:  Nikolay Kuchuk; Kateryna Sytnyk; Maxim Vasylenko; Anatolij Shakhovsky; Igor Komarnytsky; Sergei Kushnir; Yuri Gleba
Journal:  Theor Appl Genet       Date:  2006-06-07       Impact factor: 5.699

8.  Plastid transformants of tomato selected using mutations affecting ribosome structure.

Authors:  G D Nugent; M Ten Have; A van der Gulik; P J Dix; B A Uijtewaal; A P Mordhorst
Journal:  Plant Cell Rep       Date:  2005-06-18       Impact factor: 4.570

9.  Plastid transformation in the monocotyledonous cereal crop, rice (Oryza sativa) and transmission of transgenes to their progeny.

Authors:  Sa Mi Lee; Kyungsu Kang; Hyungsup Chung; Soon Hee Yoo; Xiang Ming Xu; Seung-Bum Lee; Jong-Joo Cheong; Henry Daniell; Minkyun Kim
Journal:  Mol Cells       Date:  2006-06-30       Impact factor: 5.034

10.  Plastid transformation in eggplant (Solanum melongena L.).

Authors:  A K Singh; S S Verma; K C Bansal
Journal:  Transgenic Res       Date:  2009-06-28       Impact factor: 2.788
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