Literature DB >> 12633849

Purification of active recombinant trypanosome alternative oxidase.

Coichi Nihei1, Yoshihisa Fukai, Keisuke Kawai, Arihiro Osanai, Yoshisada Yabu, Takashi Suzuki, Nobuo Ohta, Nobuko Minagawa, Kazuo Nagai, Kiyoshi Kita.   

Abstract

Trypanosome alternative oxidase (TAO) is the terminal oxidase of the respiratory chain in long slender bloodstream forms of African trypanosomes. TAO is a cytochrome-independent, cyanide-insensitive quinol oxidase. These characteristics are distinct from those of the bacterial quinol oxidases, proteins that belong to the heme-copper terminal oxidase superfamily. The inability to purify stable TAO has severely hampered biochemical studies of the alternative oxidase family. In the present study, we were able to purify recombinant TAO to homogeneity from Escherichia coli membranes using the detergent digitonin. Kinetic analysis of the purified TAO revealed that the specific inhibitor ascofuranone is a competitive inhibitor of ubiquinol oxidase activity.

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Year:  2003        PMID： 12633849     DOI： 10.1016/s0014-5793(03)00120-0

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  15 in total

Review 1.  Classical and alternative components of the mitochondrial respiratory chain in pathogenic fungi as potential therapeutic targets.

Authors:  Vicente de Paulo Martins; Taisa Magnani Dinamarco; Carlos Curti; Sérgio Akira Uyemura
Journal:  J Bioenerg Biomembr       Date:  2011-02       Impact factor: 2.945

2.  Involvement of an alternative oxidase in oxidative stress and mycelium-to-yeast differentiation in Paracoccidioides brasiliensis.

Authors:  Vicente P Martins; Taisa M Dinamarco; Frederico M Soriani; Valéria G Tudella; Sergio C Oliveira; Gustavo H Goldman; Carlos Curti; Sérgio A Uyemura
Journal:  Eukaryot Cell       Date:  2010-12-23

3.  Correlating kinetic and structural data on ubiquinone binding and reduction by respiratory complex I.

Authors:  Justin G Fedor; Andrew J Y Jones; Andrea Di Luca; Ville R I Kaila; Judy Hirst
Journal:  Proc Natl Acad Sci U S A       Date:  2017-11-13       Impact factor: 11.205

4.  Ascochlorin is a novel, specific inhibitor of the mitochondrial cytochrome bc1 complex.

Authors:  Edward A Berry; Li-Shar Huang; Dong-Woo Lee; Fevzi Daldal; Kazuo Nagai; Nobuko Minagawa
Journal:  Biochim Biophys Acta       Date:  2009-12-16

5.  Crystallization and preliminary crystallographic analysis of cyanide-insensitive alternative oxidase from Trypanosoma brucei brucei.

Authors:  Yasutoshi Kido; Tomoo Shiba; Daniel Ken Inaoka; Kimitoshi Sakamoto; Takeshi Nara; Takashi Aoki; Teruki Honma; Akiko Tanaka; Masayuki Inoue; Shigeru Matsuoka; Anthony Moore; Shigeharu Harada; Kiyoshi Kita
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-02-24

Review 6.  Targeting the alternative oxidase (AOX) for human health and food security, a pharmaceutical and agrochemical target or a rescue mechanism?

Authors:  Marten Szibor; Christina Schenkl; Mario R O Barsottini; Luke Young; Anthony L Moore
Journal:  Biochem J       Date:  2022-06-30       Impact factor: 3.766

7.  Structural and Biophysical Characterization of Purified Recombinant Arabidopsis thaliana's Alternative Oxidase 1A (rAtAOX1A): Interaction With Inhibitor(s) and Activator.

Authors:  Tadiboina Veera Sankar; Moumita Saharay; Dharawath Santhosh; Abhaypratap Vishwakarma; Kollipara Padmasree
Journal:  Front Plant Sci       Date:  2022-06-16       Impact factor: 6.627

8.  Identification of megacerotonic acid and a quinazoline derivative from Universal Natural Product Database as potential inhibitors of Trypanosoma brucei brucei alternative oxidase: molecular docking, molecular dynamic simulation and MM/PBSA analysis.

Authors:  Rahma Muhammad Adamu; Bashiru Ibrahim; Mohammed Auwal Ibrahim; Emmanuel Oluwadare Balogun
Journal:  J Biomol Struct Dyn       Date:  2021-11-23       Impact factor: 5.235

9.  Three redox states of Trypanosoma brucei alternative oxidase identified by infrared spectroscopy and electrochemistry.

Authors:  Amandine Maréchal; Yasutoshi Kido; Kiyoshi Kita; Anthony L Moore; Peter R Rich
Journal:  J Biol Chem       Date:  2009-09-19       Impact factor: 5.157

10.  Kinetic characterisation and inhibitor sensitivity of Candida albicans and Candida auris recombinant AOX expressed in a self-assembled proteoliposome system.

Authors:  Alice C Copsey; Mario R O Barsottini; Benjamin May; Fei Xu; Mary S Albury; Luke Young; Anthony L Moore
Journal:  Sci Rep       Date:  2021-07-20       Impact factor: 4.379
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