AIM: Recent studies report that the expression of cyclooxygenase (COX) in non-small cell lung cancer (NSCLC) is increased, especially in adenocarcinoma. Platelet activating factor (PAF), n-sodium butyrate (n-BT), and phorbol myristate acetate (PMA) are important mediators of the inflammatory process. METHOD: Expression of COX-2 in 67 stage 1 NSCLC paraffin-embedded tumor samples was determined by immunohistochemistry (IHC). Four NSCL cell lines were incubated and stimulated by PAF, n-BT and PMA for 48 h. Expression of COX-2 was determined by IHC, immunoblotting, and reverse transcription-polymerase chain reaction (RT-PCR). RESULT: IHC showed increasing immunoreactivity in 35 of 67 (52%) in stage I NSCLC, 31 of 53 (59%) in adenocarcinoma and 13 of 15 (87%) in bronchoalveolar cell carcinoma, but only 2 of 12 (17%) in epidermoid carcinoma. The COX-2 expression in NSCLC cells was 75% (3/4) and the COX-1 expression in NSCLC cells was 100% (4/4). After stimulation with PMA, n-BT, PAF and n-BT + PAF, the COX-2 expression in NSCLC cells was significantly increased in all cell lines. CONCLUSIONS: The expression of COX-2 in NSCLC cells is high and was up-regulated by PMA, n-BT and PAF. We consider that COX-2 inhibitors will play an important role in the therapy of NSCLC. Copyright 2002 Elsevier Science Ltd.
AIM: Recent studies report that the expression of cyclooxygenase (COX) in non-small cell lung cancer (NSCLC) is increased, especially in adenocarcinoma. Platelet activating factor (PAF), n-sodium butyrate (n-BT), and phorbol myristate acetate (PMA) are important mediators of the inflammatory process. METHOD: Expression of COX-2 in 67 stage 1 NSCLCparaffin-embedded tumor samples was determined by immunohistochemistry (IHC). Four NSCL cell lines were incubated and stimulated by PAF, n-BT and PMA for 48 h. Expression of COX-2 was determined by IHC, immunoblotting, and reverse transcription-polymerase chain reaction (RT-PCR). RESULT: IHC showed increasing immunoreactivity in 35 of 67 (52%) in stage I NSCLC, 31 of 53 (59%) in adenocarcinoma and 13 of 15 (87%) in bronchoalveolar cell carcinoma, but only 2 of 12 (17%) in epidermoid carcinoma. The COX-2 expression in NSCLC cells was 75% (3/4) and the COX-1 expression in NSCLC cells was 100% (4/4). After stimulation with PMA, n-BT, PAF and n-BT + PAF, the COX-2 expression in NSCLC cells was significantly increased in all cell lines. CONCLUSIONS: The expression of COX-2 in NSCLC cells is high and was up-regulated by PMA, n-BT and PAF. We consider that COX-2 inhibitors will play an important role in the therapy of NSCLC. Copyright 2002 Elsevier Science Ltd.
Authors: Alison L Van Dyke; Michele L Cote; Geoffrey M Prysak; Gina B Claeys; Angie S Wenzlaff; Valerie C Murphy; Fulvio Lonardo; Ann G Schwartz Journal: Carcinogenesis Date: 2008-05-02 Impact factor: 4.944
Authors: Alison L Van Dyke; Michele L Cote; Angie S Wenzlaff; Wei Chen; Judith Abrams; Susan Land; Craig N Giroux; Ann G Schwartz Journal: Cancer Epidemiol Biomarkers Prev Date: 2009-06 Impact factor: 4.254
Authors: Alison L Van Dyke; Michele L Cote; Geoffrey Prysak; Gina B Claeys; Angie S Wenzlaff; Ann G Schwartz Journal: Cancer Epidemiol Biomarkers Prev Date: 2008-01-09 Impact factor: 4.254
Authors: Ildefonso Alves da Silva-Junior; Barbara Dalmaso; Suellen Herbster; Ana Paula Lepique; Sonia Jancar Journal: Front Oncol Date: 2018-02-05 Impact factor: 6.244