Literature DB >> 12624066

Construction and evaluation of internal control DNA for PCR amplification of Chlamydia trachomatis DNA from urine samples.

Fotini Betsou1, Katy Beaumont, Jean Marie Sueur, Jeanne Orfila.   

Abstract

An internal control DNA (ICD) with the same primer binding sequences as the target Chlamydia trachomatis DNA was constructed and evaluated in a PCR assay with immunoenzymatic detection. One hundred urine specimens were tested, and 23 were found to contain inhibitors of the PCR, if not subjected to DNA extraction prior to amplification. Coamplification and detection of the ICD appeared to be a useful method for estimating the effects of inhibitors on C. trachomatis DNA amplification.

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Year:  2003        PMID： 12624066      PMCID： PMC150278          DOI： 10.1128/JCM.41.3.1274-1276.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  6 in total

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5.  Sample preparation method for polymerase chain reaction-based semiquantitative detection of Leptospira interrogans serovar hardjo subtype hardjobovis in bovine urine.

Authors:  M J Gerritsen; T Olyhoek; M A Smits; B A Bokhout
Journal:  J Clin Microbiol       Date:  1991-12       Impact factor: 5.948

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Authors:  R C Brunham; I W Maclean; B Binns; R W Peeling
Journal:  J Infect Dis       Date:  1985-12       Impact factor: 5.226
  6 in total
  4 in total

1.  Improved internal control for molecular diagnosis assays.

Authors:  T Vinayagamoorthy; Danielle Maryanski; Dilanthi Vinayagamoorthy; Katie S L Hay; Jacob Yo; Mark Carter; Joseph Wiegel
Journal:  MethodsX       Date:  2015-03-12

2.  Application of HTB-SiHa cells transfected with a recombinant plasmid for external quality assessment of Chlamydia trachomatis PCR.

Authors:  Kuo Zhang; Hong Huo; Yu Sun; Lunan Wang; Rui Zhang; Guigao Lin; Jiehong Xie; Qingtao Wang; Jinming Li
Journal:  Ann Lab Med       Date:  2014-08-21       Impact factor: 3.464

3.  Identification of two GH18 chitinase family genes and their use as targets for detection of the crayfish-plague oomycete Aphanomyces astaci.

Authors:  Gerald Hochwimmer; Reinhard Tober; Renè Bibars-Reiter; Elisabeth Licek; Ralf Steinborn
Journal:  BMC Microbiol       Date:  2009-08-31       Impact factor: 3.605

4.  A magnetic bead-based method for concentrating DNA from human urine for downstream detection.

Authors:  Hali Bordelon; Patricia K Russ; David W Wright; Frederick R Haselton
Journal:  PLoS One       Date:  2013-07-08       Impact factor: 3.240
  4 in total

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