Literature DB >> 12624059

Entirely automated quantification of human immunodeficiency virus type 1 (HIV-1) RNA in plasma by using the ultrasensitive COBAS AMPLICOR HIV-1 monitor test and RNA purification on the MagNA pure LC instrument.

Gabriele Hölzl1, Markus Stöcher, Victoria Leb, Herbert Stekel, Jörg Berg.   

Abstract

The ultrasensitive COBAS AMPLICOR HIV-1 Monitor test was complemented with automated RNA purification on the MagNA Pure LC instrument. This enabled entirely automated ultrasensitive assessment of viral loads in human immunodeficiency virus type 1 (HIV-1)-infected individuals. The detection limit of the fully automated assay and the viral load measurements in 80 clinical samples were found to be in good agreement with those of the conventional ultrasensitive COBAS AMPLICOR HIV-1 Monitor test. The fully automated assay showed markedly reduced hands-on time and was found to be suitable for the routine assessment of HIV-1 viral loads in a clinical diagnostic laboratory.

Entities:  

Mesh：

Substances：

Year:  2003        PMID： 12624059      PMCID： PMC150272          DOI： 10.1128/JCM.41.3.1248-1251.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

1.  Fully automated nucleic acid extraction: MagNA Pure LC.

Authors:  H H Kessler; G Mühlbauer; E Stelzl; E Daghofer; B I Santner; E Marth
Journal:  Clin Chem       Date:  2001-06       Impact factor: 8.327

2.  Detection of herpes simplex virus DNA in genital and dermal specimens by LightCycler PCR after extraction using the IsoQuick, MagNA Pure, and BioRobot 9604 methods.

Authors:  M J Espy; P N Rys; A D Wold; J R Uhl; L M Sloan; G D Jenkins; D M Ilstrup; F R Cockerill; R Patel; J E Rosenblatt; T F Smith
Journal:  J Clin Microbiol       Date:  2001-06       Impact factor: 5.948

3.  Quantification of fungal DNA by using fluorescence resonance energy transfer and the light cycler system.

Authors:  J Loeffler; N Henke; H Hebart; D Schmidt; L Hagmeyer; U Schumacher; H Einsele
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

4.  Prevalence of human immunodeficiency virus type 1 (HIV-1) non-B subtypes in foreigners living in Madrid, Spain, and comparison of the performances of the AMPLICOR HIV-1 MONITOR version 1.0 and the new automated version 1.5.

Authors:  A Holguín; B Aracil; A Alvarez; C Barros; V Soriano
Journal:  J Clin Microbiol       Date:  2001-05       Impact factor: 5.948

5.  Highly active antiretroviral therapy decreases mortality and morbidity in patients with advanced HIV disease.

Authors:  E L Murphy; A C Collier; L A Kalish; S F Assmann; M F Para; T P Flanigan; P N Kumar; L Mintz; F R Wallach; G J Nemo
Journal:  Ann Intern Med       Date:  2001-07-03       Impact factor: 25.391

6.  COBAS AMPLICOR: fully automated RNA and DNA amplification and detection system for routine diagnostic PCR.

Authors:  N DiDomenico; H Link; R Knobel; T Caratsch; W Weschler; Z G Loewy; M Rosenstraus
Journal:  Clin Chem       Date:  1996-12       Impact factor: 8.327

7.  Predictive value of the ultrasensitive HIV viral load assay in clinical practice.

Authors:  D Muir; D White; J King; N Verlander; D Pillay
Journal:  J Med Virol       Date:  2000-08       Impact factor: 2.327

8.  Treatment with indinavir, zidovudine, and lamivudine in adults with human immunodeficiency virus infection and prior antiretroviral therapy.

Authors:  R M Gulick; J W Mellors; D Havlir; J J Eron; C Gonzalez; D McMahon; D D Richman; F T Valentine; L Jonas; A Meibohm; E A Emini; J A Chodakewitz
Journal:  N Engl J Med       Date:  1997-09-11       Impact factor: 91.245

Review 9.  Automation of laboratory testing for infectious diseases using the polymerase chain reaction-- our past, our present, our future.

Authors:  D Jungkind
Journal:  J Clin Virol       Date:  2001-01       Impact factor: 3.168

Review 10.  Antiretroviral treatment for adult HIV infection in 2002: updated recommendations of the International AIDS Society-USA Panel.

Authors:  Patrick G Yeni; Scott M Hammer; Charles C J Carpenter; David A Cooper; Margaret A Fischl; Jose M Gatell; Brian G Gazzard; Martin S Hirsch; Donna M Jacobsen; David A Katzenstein; Julio S G Montaner; Douglas D Richman; Michael S Saag; Mauro Schechter; Robert T Schooley; Melanie A Thompson; Stefano Vella; Paul A Volberding
Journal:  JAMA       Date:  2002-07-10       Impact factor: 56.272
View more
  7 in total

1.  Need for procedural details in the protocol for specimen processing by the MagNA Pure LC Instrument.

Authors:  Joseph D C Yao; Jeffrey Germer; Robin Patel
Journal:  J Clin Microbiol       Date:  2003-10       Impact factor: 5.948

Review 2.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

3.  Genotypic prediction of human immunodeficiency virus type 1 tropism by use of plasma and peripheral blood mononuclear cells in the routine clinical laboratory.

Authors:  Christian Paar; Maria Geit; Herbert Stekel; Jörg Berg
Journal:  J Clin Microbiol       Date:  2011-05-18       Impact factor: 5.948

4.  Evaluation of automated sample preparation and quantitative PCR LCx assay for determination of human immunodeficiency virus type 1 RNA.

Authors:  Zsofia Muller; Evelyn Stelzl; Michael Bozic; Josef Haas; Egon Marth; Harald H Kessler
Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948

5.  Field evaluation of Abbott Real Time HIV-1 Qualitative test for early infant diagnosis using dried blood spots samples in comparison to Roche COBAS Ampliprep/COBAS TaqMan HIV-1 Qual test in Kenya.

Authors:  Joy Chang; Kenneth Omuomo; Emily Anyango; Leonard Kingwara; Frank Basiye; Alex Morwabe; Vedapuri Shanmugam; Shon Nguyen; Jennifer Sabatier; Clement Zeh; Dennis Ellenberger
Journal:  J Virol Methods       Date:  2014-04-13       Impact factor: 2.014

6.  Genotypic antiretroviral resistance testing for human immunodeficiency virus type 1 integrase inhibitors by use of the TruGene sequencing system.

Authors:  Christian Paar; Christina Palmetshofer; Karin Flieger; Maria Geit; Rolf Kaiser; Herbert Stekel; Jörg Berg
Journal:  J Clin Microbiol       Date:  2008-10-22       Impact factor: 5.948

7.  Plasma load discrepancies between the Roche Cobas Amplicor human immunodeficiency virus type 1 (HIV-1) Monitor version 1.5 and Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 assays.

Authors:  Joseph D C Yao; Jeffrey J Germer; F Damond; B Roquebert; D Descamps
Journal:  J Clin Microbiol       Date:  2008-02       Impact factor: 5.948
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.