Literature DB >> 12620880

Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR.

Loree C Heller1, Carisa R Davis, K Kealy Peak, David Wingfield, Andrew C Cannons, Philip T Amuso, Jacqueline Cattani.   

Abstract

In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

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Year:  2003        PMID： 12620880      PMCID： PMC150052          DOI： 10.1128/AEM.69.3.1844-1846.2003

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  6 in total

1.  Continuous fluorescence monitoring of rapid cycle DNA amplification.

Authors:  C T Wittwer; M G Herrmann; A A Moss; R P Rasmussen
Journal:  Biotechniques       Date:  1997-01       Impact factor: 1.993

2.  ANALYZING TABLES OF STATISTICAL TESTS.

Authors:  William R Rice
Journal:  Evolution       Date:  1989-01       Impact factor: 3.694

3.  Effect of stress treatments on the detection of Listeria monocytogenes and enterotoxigenic Escherichia coli by the polymerase chain reaction.

Authors:  C I Masters; J A Shallcross; B M Mackey
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4.  A large community outbreak of salmonellosis caused by intentional contamination of restaurant salad bars.

Authors:  T J Török; R V Tauxe; R P Wise; J R Livengood; R Sokolow; S Mauvais; K A Birkness; M R Skeels; J M Horan; L R Foster
Journal:  JAMA       Date:  1997-08-06       Impact factor: 56.272

5.  Polymerase chain reaction detection of nonviable bacterial pathogens.

Authors:  K L Josephson; C P Gerba; I L Pepper
Journal:  Appl Environ Microbiol       Date:  1993-10       Impact factor: 4.792

6.  An outbreak of Shigella dysenteriae type 2 among laboratory workers due to intentional food contamination.

Authors:  S A Kolavic; A Kimura; S L Simons; L Slutsker; S Barth; C E Haley
Journal:  JAMA       Date:  1997-08-06       Impact factor: 56.272
  6 in total
  4 in total

1.  Comparison of methods of extracting Salmonella enterica serovar Enteritidis DNA from environmental substrates and quantification of organisms by using a general internal procedural control.

Authors:  M M Klerks; A H C van Bruggen; C Zijlstra; M Donnikov
Journal:  Appl Environ Microbiol       Date:  2006-06       Impact factor: 4.792

2.  Prevalence and characterization of shiga toxin-producing Escherichia coli in swine feces recovered in the National Animal Health Monitoring System's Swine 2000 study.

Authors:  Pina M Fratamico; Lori K Bagi; Eric J Bush; Barbara T Solow
Journal:  Appl Environ Microbiol       Date:  2004-12       Impact factor: 4.792

3.  Evaluation of PCR inhibitory effect of enrichment broths and comparison of DNA extraction methods for detection of Salmonella Enteritidis using real-time PCR assay.

Authors:  Ji Yeon Hyeon; In Gyun Hwang; Hyo Sun Kwak; Chankyu Park; In Soo Choi; Kun Ho Seo
Journal:  J Vet Sci       Date:  2010-06       Impact factor: 1.672

4.  A simple and rapid procedure for the detection of genes encoding Shiga toxins and other specific DNA sequences.

Authors:  Bożena Nejman-Faleńczyk; Sylwia Bloch; Aleksandra Januszkiewicz; Alicja Węgrzyn; Grzegorz Węgrzyn
Journal:  Toxins (Basel)       Date:  2015-11-13       Impact factor: 4.546
  4 in total

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