| Literature DB >> 12617061 |
Prem Kumarathasan1, Renaud Vincent.
Abstract
New high-performance liquid chromatographic (HPLC) methods with amperometric-CoulArray detection were developed for simultaneous analyses of norepinephrine, epinephrine, L-DOPA, dopamine, 3-nitrotyrosine, m-, o-, and p-tyrosines. Overall, detection limit was in the low pmol range with amperometry, and in the low fmol range for the CoulArray method. Linear (r2 = 0.99) detector performances were observed in the ranges of 2-200 pmol with amperometry, and 0.2-20 pmol for the CoulArray method. Analytical precision values were better than 80 and 95% for HPLC-amperometry and HPLC-CoulArray method, respectively. These methods offer sensitivity, specificity, minimal sample requirement, and especially the HPLC-CoulArray method allows simultaneous assessment of various similar biomolecules.Entities:
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Year: 2003 PMID： 12617061 DOI： 10.1016/s0021-9673(02)01598-4
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759