BACKGROUND: The conventional deuterated-retinol-dilution (DRD) technique provides a quantitative estimate of total body stores of vitamin A in humans. The procedure requires equilibration of serum deuterated retinol with nondeuterated retinol after administration of an oral dose of deuterated vitamin A. Equilibration takes approximately 3 wk to complete. OBJECTIVE: Our goal was to develop a predictive mathematical formula for quantitative assessment of total body stores of vitamin A in adults by using a procedure that takes less time to perform because serum isotope equilibration is not required, so that blood drawing can be done 3 d, instead of approximately 3 wk, after isotope dosing. DESIGN: Ratios of serum deuterated to nondeuterated retinol (D:H retinol) were determined in Filipino adults (n = 68) 3 and 20 d after an oral dose of 0.015 mmol [(2)H(4)]retinyl acetate and in Guatemalan adults (n = 15) 3 and 21 d after a 0.030-mmol dose. D:H retinol values 20 or 21 d after the isotope dose were used in a mathematical formula to obtain quantitative estimates of total body stores of vitamin A that were then correlated with serum D:H retinol values 3 d after the isotope dose. RESULTS: The relation between these variables was nonlinear and was described by the following equation: total body stores of vitamin A (in mmol retinol) = 0.00468 x 10(37(isotope dose in mmol))/D:H retinol in serum 3 d after the isotope dose. CONCLUSION: A 3-d DRD technique could be used for quantitative assessment of total body stores of vitamin A; this technique takes less time than does the conventional DRD technique.
BACKGROUND: The conventional deuterated-retinol-dilution (DRD) technique provides a quantitative estimate of total body stores of vitamin A in humans. The procedure requires equilibration of serum deuterated retinol with nondeuterated retinol after administration of an oral dose of deuterated vitamin A. Equilibration takes approximately 3 wk to complete. OBJECTIVE: Our goal was to develop a predictive mathematical formula for quantitative assessment of total body stores of vitamin A in adults by using a procedure that takes less time to perform because serum isotope equilibration is not required, so that blood drawing can be done 3 d, instead of approximately 3 wk, after isotope dosing. DESIGN: Ratios of serum deuterated to nondeuterated retinol (D:H retinol) were determined in Filipino adults (n = 68) 3 and 20 d after an oral dose of 0.015 mmol [(2)H(4)]retinyl acetate and in Guatemalan adults (n = 15) 3 and 21 d after a 0.030-mmol dose. D:H retinol values 20 or 21 d after the isotope dose were used in a mathematical formula to obtain quantitative estimates of total body stores of vitamin A that were then correlated with serum D:H retinol values 3 d after the isotope dose. RESULTS: The relation between these variables was nonlinear and was described by the following equation: total body stores of vitamin A (in mmol retinol) = 0.00468 x 10(37(isotope dose in mmol))/D:H retinol in serum 3 d after the isotope dose. CONCLUSION: A 3-d DRD technique could be used for quantitative assessment of total body stores of vitamin A; this technique takes less time than does the conventional DRD technique.
Authors: Bryan M Gannon; Ashley R Valentine; Christopher R Davis; Julie A Howe; Sherry A Tanumihardjo Journal: J Nutr Date: 2018-08-01 Impact factor: 4.798
Authors: Jean F Bationo; Augustin N Zeba; Nadine D Coulibaly; Jesse Sheftel; Christopher R Davis; Imael H N Bassole; Nicolas Barro; Jean B Ouedraogo; Sherry A Tanumihardjo Journal: Exp Biol Med (Maywood) Date: 2019-09-23
Authors: Luigi Ferrucci; John R B Perry; Amy Matteini; Markus Perola; Toshiko Tanaka; Kaisa Silander; Neil Rice; David Melzer; Anna Murray; Christie Cluett; Linda P Fried; Demetrius Albanes; Anna-Maria Corsi; Antonio Cherubini; Jack Guralnik; Stefania Bandinelli; Andrew Singleton; Jarmo Virtamo; Jeremy Walston; Richard D Semba; Timothy M Frayling Journal: Am J Hum Genet Date: 2009-01-29 Impact factor: 11.025
Authors: Rachel E Kopec; Jessica L Cooperstone; Ralf M Schweiggert; Gregory S Young; Earl H Harrison; David M Francis; Steven K Clinton; Steven J Schwartz Journal: J Nutr Date: 2014-06-04 Impact factor: 4.798