Literature DB >> 12586374

Assembly and overexpression of membrane proteins in Escherichia coli.

David Drew1, Linda Fröderberg, Louise Baars, Jan-Willem L de Gier.   

Abstract

The bacterium Escherichia coli is one of the most popular model systems to study the assembly of membrane proteins of the so-called helix-bundle class. Here, based on this system, we review and discuss what is currently known about the assembly of these membrane proteins. In addition, we will briefly review and discuss how E. coli has been used as a vehicle for the overexpression of membrane proteins. Copyright 2003 Elsevier Science B.V.

Entities:  

Mesh:

Substances:

Year:  2003        PMID: 12586374     DOI: 10.1016/s0005-2736(02)00707-1

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN: 0006-3002


  40 in total

1.  Reprogramming chaperone pathways to improve membrane protein expression in Escherichia coli.

Authors:  Brent L Nannenga; François Baneyx
Journal:  Protein Sci       Date:  2011-07-07       Impact factor: 6.725

2.  Towards higher-throughput membrane protein production for structural genomics initiatives.

Authors:  Philip D Laible; Heather N Scott; Lynda Henry; Deborah K Hanson
Journal:  J Struct Funct Genomics       Date:  2004

3.  Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator-repressor interactions.

Authors:  Anoop Narayanan; Marc Ridilla; Dinesh A Yernool
Journal:  Protein Sci       Date:  2011-01       Impact factor: 6.725

4.  Large-scale expression and purification of a G-protein-coupled receptor for structure determination -- an overview.

Authors:  Reinhard Grisshammer; Jim F White; Loc B Trinh; Joseph Shiloach
Journal:  J Struct Funct Genomics       Date:  2005

5.  Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.

Authors:  Shawn Lewenza; Jennifer L Gardy; Fiona S L Brinkman; Robert E W Hancock
Journal:  Genome Res       Date:  2005-02       Impact factor: 9.043

6.  Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen.

Authors:  Nicolas André; Nadia Cherouati; Cécile Prual; Tania Steffan; Gabrielle Zeder-Lutz; Thierry Magnin; Franc Pattus; Hartmut Michel; Renaud Wagner; Christoph Reinhart
Journal:  Protein Sci       Date:  2006-04-05       Impact factor: 6.725

7.  Engineering membrane protein overproduction in Escherichia coli.

Authors:  Daniel Martinez Molina; Tobias Cornvik; Said Eshaghi; Jesper Z Haeggström; Pär Nordlund; Marina Ignatushchenko Sabet
Journal:  Protein Sci       Date:  2008-02-27       Impact factor: 6.725

8.  Tuning Escherichia coli for membrane protein overexpression.

Authors:  Samuel Wagner; Mirjam M Klepsch; Susan Schlegel; Ansgar Appel; Roger Draheim; Michael Tarry; Martin Högbom; Klaas J van Wijk; Dirk J Slotboom; Jan O Persson; Jan-Willem de Gier
Journal:  Proc Natl Acad Sci U S A       Date:  2008-09-16       Impact factor: 11.205

9.  Quality control of overexpressed membrane proteins.

Authors:  Eric R Geertsma; Maarten Groeneveld; Dirk-Jan Slotboom; Bert Poolman
Journal:  Proc Natl Acad Sci U S A       Date:  2008-04-07       Impact factor: 11.205

10.  High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1.

Authors:  Hui Ren; Daoyong Yu; Baosheng Ge; Brian Cook; Zhinan Xu; Shuguang Zhang
Journal:  PLoS One       Date:  2009-02-18       Impact factor: 3.240

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.