Literature DB >> 1254927

Flow microfluorometric system for screening gynecologic cytology specimens using propidium iodide-fluorescein isothiocyanate.

B J Fowlkes, C J Herman, M Cassidy.   

Abstract

Seventy cervical cytology specimens have been screened by a xero resolution flow analyzer-sorter using propidium iodide and fluorescein isothiocyanate as fluorochromes for nucleus and cytoplasm, respectively. This system shows a 1% sensitivity for detection of abnormal cells using only crude visual data analysis. Screening of clinical specimens was performed on the instrument with a 5.8% false negative rate and a 11.8% false positive rate by comparison with routine visual cytologic evaluation of the same samples.

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Year:  1976        PMID： 1254927     DOI： 10.1177/24.1.1254927

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  4 in total

1.  Quantitative determination of transformed cells in a mixed population by stimultaneous fluorescence analysis of cell surface and DNA an individual cells.

Authors:  S P Hawkes; J C Bartholomew
Journal:  Proc Natl Acad Sci U S A       Date:  1977-04       Impact factor: 11.205

2.  Effects of DTIC, DM-COOK and ICRF-159 on the number of circulating Lewis lung carcinoma cells detected by flow cytometry.

Authors:  T Giraldi; G Sava; R Cherubino; G Bottiroli; G Mazzini
Journal:  Clin Exp Metastasis       Date:  1984 Apr-Jun       Impact factor: 5.150

3.  Flowcytometric and cytogenetic analysis of human cultured cell lines derived from high- and low-grade astrocytomas.

Authors:  N Shitara; P E McKeever; J Whang-Peng; T Knutsen; B H Smith; P L Kornblith
Journal:  Acta Neuropathol       Date:  1983       Impact factor: 17.088

4.  The in vitro effect of electromagnetically generated shock waves (Lithostar) on the Dunning R3327 PAT-2 rat prostatic cancer cell-line. A potentiating effect on the in vitro cytotoxicity of vinblastin.

Authors:  G O Oosterhof; G A Smits; J E de Ruyter; R J van Moorselaar; J A Schalken; F M Debruyne
Journal:  Urol Res       Date:  1989
  4 in total

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