PURPOSE: To detect endogenous substance P (SP) and neurokinin receptor 1 (NK1R) in cultured human corneal epithelial cells (HE) and cultured human keratocytes (HK). METHODS: Messenger RNA (mRNA) expression of SP and endogenous SP in HE and HK was investigated by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay. mRNA expression of NK1R in HE and HK was investigated by RT-PCR. RESULTS: The mRNA expression of SP and endogenous SP were recognized in HE and HK. Furthermore, the mRNA of NK1R was expressed in HE and HK. CONCLUSION: It was suggested that endogenous SP regulates the biological functions of HE and HK in autocrine or paracrine fashion.
PURPOSE: To detect endogenous substance P (SP) and neurokinin receptor 1 (NK1R) in cultured human corneal epithelial cells (HE) and cultured human keratocytes (HK). METHODS: Messenger RNA (mRNA) expression of SP and endogenous SP in HE and HK was investigated by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay. mRNA expression of NK1R in HE and HK was investigated by RT-PCR. RESULTS: The mRNA expression of SP and endogenous SP were recognized in HE and HK. Furthermore, the mRNA of NK1R was expressed in HE and HK. CONCLUSION: It was suggested that endogenous SP regulates the biological functions of HE and HK in autocrine or paracrine fashion.
Authors: Alireza Mashaghi; Anna Marmalidou; Mohsen Tehrani; Peter M Grace; Charalabos Pothoulakis; Reza Dana Journal: Cell Mol Life Sci Date: 2016-06-17 Impact factor: 9.261
Authors: Jennifer M Brazill; Alec T Beeve; Clarissa S Craft; Jason J Ivanusic; Erica L Scheller Journal: J Bone Miner Res Date: 2019-07-26 Impact factor: 6.741