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Literature DB >> 12531508

Removal of PCR inhibitors from soil DNA by chemical flocculation.

Michael D Braid¹, Laura M Daniels, Christopher L Kitts.

Abstract

Extracting high-purity DNA directly from soil has become essential for the study of microorganisms in environmental samples. However, many soils contain compounds that inhibit enzymes involved in manipulating DNA. In this study, chemical flocculation using multivalent cations was investigated as a potential method for eliminating soil-based inhibitors during the extraction process. The addition of AlNH(4)(SO(4))(2) during extraction significantly reduced the co-purification of PCR inhibitors with minimal loss of DNA yield.

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Year: 2003 PMID： 12531508 DOI： 10.1016/s0167-7012(02)00210-5

Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363

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Cited

40 in total

1. Practical method for extraction of PCR-quality DNA from environmental soil samples.

Authors: Kelly A Fitzpatrick; Gilbert J Kersh; Robert F Massung
Journal: Appl Environ Microbiol Date: 2010-04-30 Impact factor: 4.792

2. Phylogenetic and multivariate analyses to determine the effects of different tillage and residue management practices on soil bacterial communities.

Authors: Javier A Ceja-Navarro; Flor N Rivera-Orduña; Leonardo Patiño-Zúñiga; Antón Vila-Sanjurjo; José Crossa; Bram Govaerts; Luc Dendooven
Journal: Appl Environ Microbiol Date: 2010-04-09 Impact factor: 4.792

3. Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors.

Authors: Jianlin Jiang; Kerri A Alderisio; Ajaib Singh; Lihua Xiao
Journal: Appl Environ Microbiol Date: 2005-03 Impact factor: 4.792

4. Dynamics of a pig slurry microbial community during anaerobic storage and management.

Authors: Pascal Peu; Hubert Brugère; Anne-Marie Pourcher; Monique Kérourédan; Jean-Jacques Godon; Jean-Philippe Delgenès; Patrick Dabert
Journal: Appl Environ Microbiol Date: 2006-05 Impact factor: 4.792

5. PCR inhibitor levels in concentrates of biosolid samples predicted by a new method based on excitation-emission matrix spectroscopy.

Authors: Channah Rock; Absar Alum; Morteza Abbaszadegan
Journal: Appl Environ Microbiol Date: 2010-10-22 Impact factor: 4.792

Removal of PCR inhibitors from soil DNA by chemical flocculation.

1. Practical method for extraction of PCR-quality DNA from environmental soil samples.

2. Phylogenetic and multivariate analyses to determine the effects of different tillage and residue management practices on soil bacterial communities.

3. Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors.

4. Dynamics of a pig slurry microbial community during anaerobic storage and management.

5. PCR inhibitor levels in concentrates of biosolid samples predicted by a new method based on excitation-emission matrix spectroscopy.

6. Bacterial diversity and activity along a salinity gradient in soda lakes of the Kulunda Steppe (Altai, Russia).

7. Influence of algal community structure on denitrification rates in periphyton cultivated on artificial substrata.

8. Innovative methods for soil DNA purification tested in soils with widely differing characteristics.

9. Widespread occurrence of bacterial human virulence determinants in soil and freshwater environments.

10. On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System.