Expression of bcl(xs) and c-myc in atretic follicles of mouse ovary.

At birth, in the human female, one million follicles are present, out of which only 450 ovulate during the reproductive lifespan while the remainder of the 99.9% follicles degenerate. It is therefore important to understand the regulation of follicular atresia. Immature mice injected with pregnant mare serum gonadotrophin (PMSG) were used as a model for follicular atresia. In this model, we demonstrated by various methods, including the TdT-mediated dUTP nick end labelling (TUNEL) technique, that granulosa cells in atretic follicles undergo apoptosis. In eukaryotic cells, apoptosis is regulated by genes such as bcl(2) and c-myc. Regulation of apoptosis in the ovary by these proteins/genes was studied using the mouse model. bcl(2) is anti-apoptotic; however, bcl(xs), a member of the bcl(2) family, is apoptotic. Immunohistochemical localization of bcl(xs) in the granulosa cells of atretic follicles suggested its role in follicular atresia. Expression of c-myc was studied by in-situ hybridization in the mouse follicular atresia model. In the atretic follicles, expression of c-myc in granulosa cells was observed. Thus our studies indicate that bcl(xs) regulates granulosa cell apoptosis, while c-myc may also play a role in cell death during follicular atresia.