| Literature DB >> 12509997 |
Elzbieta Hiszczyńska-Sawicka1, Anna Brillowska-Dabrowska, Sławomir Dabrowski, Halina Pietkiewicz, Przemysław Myjak, Józef Kur.
Abstract
This report describes a simple, highly efficient and reproducible method for obtaining large quantities of highly pure recombinant Toxoplasma gondii antigens, which can be used for diagnostic application. The obtained T. gondii SAG1, GRA1, and GRA7 antigens (as fusion proteins), expressed in Escherichia coli, contained polyhistidine tags at the N- and C-ends that allowed single-step isolation by metal-affinity chromatography on Ni(2+)-IDA-Sepharose columns. The immunoreactivity of the recombinant antigens was tested in an enzyme-linked immunosorbent assay (ELISA) format for potential application in the serodiagnosis of T. gondii infection.Entities:
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Year: 2003 PMID： 12509997 DOI： 10.1016/s1046-5928(02)00593-4
Source DB: PubMed Journal: Protein Expr Purif ISSN： 1046-5928 Impact factor: 1.650