Literature DB >> 1249069

Polypeptide halomethyl ketones bind to serine proteases as analogs of the tetrahedral intermediate. X-ray crystallographic comparison of lysine- and phenylalanine-polypeptide chloromethyl ketone-inhibited subtilisin.

T L Poulos, R A Alden, S T Freer, J J Birktoft, J Kraut.   

Abstract

1. A detailed study of cytochrome C oxidse activity with Keilin-Hartree particles and purified beef heart enzyme, at low ionic strength and low cytochrome C concentrations, showed biphasic kinetics with apparent Km1 = 5 x 10(-8) M, and apparent Km2 = 0.35 to 1.0 x 10(-6) M. Direct binding studies with purified oxidase, phospholipid-containing as well as phospholipid-depleted, demonstrated two sites of interaction of cytochrome c with the enzyme, with KD2 less than or equal to 10(-7) M, and KD2 = 10(-6) M. 2...

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Year:  1976        PMID： 1249069

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  17 in total

1.  Structure of neurolysin reveals a deep channel that limits substrate access.

Authors:  C K Brown; K Madauss; W Lian; M R Beck; W D Tolbert; D W Rodgers
Journal:  Proc Natl Acad Sci U S A       Date:  2001-03-06       Impact factor: 11.205

2.  Crystallographic and kinetic investigations of the covalent complex formed by a specific tetrapeptide aldehyde and the serine protease from Streptomyces griseus.

Authors:  G D Brayer; L T Delbaere; M N James; C A Bauer; R C Thompson
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205

3.  Structure of human neutrophil elastase in complex with a peptide chloromethyl ketone inhibitor at 1.84-A resolution.

Authors:  M A Navia; B M McKeever; J P Springer; T Y Lin; H R Williams; E M Fluder; C P Dorn; K Hoogsteen
Journal:  Proc Natl Acad Sci U S A       Date:  1989-01       Impact factor: 11.205

4.  A 13C-NMR study of the role of Asn-155 in stabilizing the oxyanion of a subtilisin tetrahedral adduct.

Authors:  T P O'connell; R M Day; E V Torchilin; W W Bachovchin; J G Malthouse
Journal:  Biochem J       Date:  1997-09-15       Impact factor: 3.857

5.  Designing substrate specificity by protein engineering of electrostatic interactions.

Authors:  J A Wells; D B Powers; R R Bott; T P Graycar; D A Estell
Journal:  Proc Natl Acad Sci U S A       Date:  1987-03       Impact factor: 11.205

6.  Nerve growth factor. A structural relationship between its proteolytic and leukocyte-chemotactic active sites.

Authors:  M Young; A P Gee; M D Boyle; M J Lawman; K L Munger
Journal:  Mol Cell Biochem       Date:  1985-02       Impact factor: 3.396

7.  The antibacterial activity of LL-37 against Treponema denticola is dentilisin protease independent and facilitated by the major outer sheath protein virulence factor.

Authors:  Graciela Rosen; Michael N Sela; Gilad Bachrach
Journal:  Infect Immun       Date:  2011-12-19       Impact factor: 3.441

Review 8.  Structural basis of substrate specificity in the serine proteases.

Authors:  J J Perona; C S Craik
Journal:  Protein Sci       Date:  1995-03       Impact factor: 6.725

9.  Mechanism of the reaction of papain with substrate-derived diazomethyl ketones. Implications for the difference in site specificity of halomethyl ketones for serine proteinases and cysteine proteinases and for stereoelectronic requirements in the papain catalytic mechanism.

Authors:  K Brocklehurst; J P Malthouse
Journal:  Biochem J       Date:  1978-11-01       Impact factor: 3.857

10.  Site-directed mutagenesis and the role of the oxyanion hole in subtilisin.

Authors:  P Bryan; M W Pantoliano; S G Quill; H Y Hsiao; T Poulos
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205
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