Literature DB >> 1248332

In situ hybridization of "nick-translated" 3H-ribosomal DNA to chromosomes from salamanders.

H C Macgregor, S Mizuno.   

Abstract

A technique is described for preparation of 3H-labelled DNA by nick-translation employing deoxyribonuclease I and DNA polymerase I. The labelled DNA can be obtained in high yield with specific activities of 10(6) cpm/mug or more. Ribosomal DNA, isolated from ovaries of young Xenopus laevis, and whole DNA from Plethodon cinereus were labelled in this way. The rDNA was used for in situ hybridization to meiotic chromosomes from P. cinereus, P. vehiculum and P. dunni. Autoradiographs of in situ hybrids were exposed for 5 to 10 days, by which time nucleolus organizer regions on the chromosomes of all 3 species were clearly and specifically labelled. In all cases, labelling was confined to a short region near the middle of the short arm of both halves of a medium length bivalent. It is concluded that nick-translation is a useful and altogether efficient method of labelling nucleic acids for subsequent use in experiments involving in situ hybridizations.

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Year:  1976        PMID： 1248332     DOI： 10.1007/bf00331829

Source DB:  PubMed          Journal:  Chromosoma        ISSN： 0009-5915            Impact factor:   4.316


  11 in total

1.  In situ hybridization of ribosomal DNA labelled with 125iodine to metaphase and lampbrush chromosomes from newts.

Authors:  S Hennen; S Mizuno; H C Macgregor
Journal:  Chromosoma       Date:  1975       Impact factor: 4.316

2.  A quick-freeze method for making smear slides permanent.

Authors:  A D CONGER; L M FAIRCHILD
Journal:  Stain Technol       Date:  1953-11

3.  The radioiodination of RNA and DNA to high specific activities.

Authors:  W Prensky
Journal:  Methods Cell Biol       Date:  1976       Impact factor: 1.441

4.  Chromosomes, DNA sequences, and evolution in salamanders of the genus Plethodon.

Authors:  S Mizuno; H C Macgregor
Journal:  Chromosoma       Date:  1974       Impact factor: 4.316

5.  The nucleolar organizer of Plethodon cinereus cinereus (Green). I. Location of the nucleolar organizer by in situ nucleic acid hybridization.

Authors:  H C Macgregor; J Kezer
Journal:  Chromosoma       Date:  1973-07-18       Impact factor: 4.316

6.  Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break.

Authors:  R B Kelly; N R Cozzarelli; M P Deutscher; I R Lehman; A Kornberg
Journal:  J Biol Chem       Date:  1970-01-10       Impact factor: 5.157

7.  Gene linkage by RNA-DNA hybridization. II. Arrangement of the redundant gene sequences for 28 s and 18 s ribosomal RNA.

Authors:  D D Brown; C S Weber
Journal:  J Mol Biol       Date:  1968-06-28       Impact factor: 5.469

8.  Characterization of six cloned DNAs from Drosophila melanogaster, including one that contains the genes for rRNA.

Authors:  D M Glover; R L White; D J Finnegan; D S Hogness
Journal:  Cell       Date:  1975-06       Impact factor: 41.582

9.  A fresh look at meiosis and centromeric heterochromatin in the red-backed salamander, Plethodon cinereus cinereus (Green).

Authors:  J Kezer; H C Macgregor
Journal:  Chromosoma       Date:  1971       Impact factor: 4.316

10.  Nucleotide sequence of the rightward operator of phage lambda.

Authors:  T Maniatis; A Jeffrey; D G Kleid
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205
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  34 in total

1.  Structure and chromosomal localization of DNA sequences related to ribosomal subrepeats in Vicia faba.

Authors:  F Maggini; R Cremonini; C Zolfino; G F Tucci; R D'Ovidio; V Delre; C DePace; G T Scarascia Mugnozza; P G Cionini
Journal:  Chromosoma       Date:  1991-05       Impact factor: 4.316

Review 2.  Evolutionary cytogenetics in salamanders.

Authors:  Stanley K Sessions
Journal:  Chromosome Res       Date:  2008       Impact factor: 5.239

3.  Plasmid-like DNA is part of mitochondrial DNA in Podospora anserina.

Authors:  U Kück; U Stahl; K Esser
Journal:  Curr Genet       Date:  1981-05       Impact factor: 3.886

4.  Cytological localization of thePGIP genes in the embryo suspensor cells ofPhaseolus vulgavis L.

Authors:  M Frediani; R Cremonini; G Salvi; C Caprari; A Desiderio; R D'Ovidio; F Cervone; G De Lorenzo
Journal:  Theor Appl Genet       Date:  1993-11       Impact factor: 5.699

5.  Legumin of Vicia faba major: accumulation in developing cotyledons, purification, mRNA characterization and chromosomal location of coding genes.

Authors:  C De Pace; V Delre; G T Scarascia Mugnozza; F Maggini; R Cremonini; M Frediani; P G Cionini
Journal:  Theor Appl Genet       Date:  1991-11       Impact factor: 5.699

6.  Variation within and between nucleolar organizer regions in Australian hylid frogs (Anura) shown by 18S + 28S in-situ hybridization.

Authors:  M King; N Contreras; R L Honeycutt
Journal:  Genetica       Date:  1990       Impact factor: 1.082

7.  Chromosome banding in amphibia. XI. Constitutive heterochromatin, nucleolus organizers, 18S + 28S and 5S ribosomal RNA genes in Ascaphidae, Pipidae, Discoglossidae and Pelobatidae.

Authors:  M Schmid; L Vitelli; R Batistoni
Journal:  Chromosoma       Date:  1987       Impact factor: 4.316

8.  Herbert Macgregor (1933-2018).

Authors:  Joseph G Gall
Journal:  Chromosome Res       Date:  2018-10-01       Impact factor: 5.239

9.  Spacer size heterogeneity in ribosomal DNA of Chironomus thummi is due to a 120 bp repeat homologous to a predominantly centromeric repeated sequence.

Authors:  N Israelewski; E R Schmidt
Journal:  Nucleic Acids Res       Date:  1982-12-11       Impact factor: 16.971

10.  Cytological evidence of transcription of highly repeated DNA sequences during the lampbrush stage in Triturus cristatus carnifex.

Authors:  J M Varley; H C Macgregor; I Nardi; C Andrews; H P Erba
Journal:  Chromosoma       Date:  1980       Impact factor: 4.316
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