Literature DB >> 12466562

Polyamine-assisted rapid and clean cleavage of oligonucleotides from cis-diol bearing universal support.

P Kumar1, G Dhawan, R Chandra, K C Gupta.   

Abstract

Polyamine-assisted deprotection conditions have been developed for the rapid and clean cleavage of oligonucleotide chains from a cis-diol group bearing universal polymer support, making it compatible with modern oligonucleotide synthesis via all types of phosphoramidite synthons, including base labile protecting group bearing synthons as well. The synthesized oligonucleotides were found to be comparable with the corresponding standard oligomers with respect to their retention time on HPLC, mass on MALDI-TOF and biological activity in PCR amplification.

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Year:  2002        PMID： 12466562      PMCID： PMC137985          DOI： 10.1093/nar/gnf130

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  10 in total

1.  Hydroquinone-O,O'-diacetic acid ('Q-linker') as a replacement for succinyl and oxalyl linker arms in solid phase oligonucleotide synthesis.

Authors:  R T Pon; S Yu
Journal:  Nucleic Acids Res       Date:  1997-09-15       Impact factor: 16.971

2.  A new universal linker for solid phase DNA synthesis.

Authors:  M H Lyttle; D Hudson; R M Cook
Journal:  Nucleic Acids Res       Date:  1996-07-15       Impact factor: 16.971

3.  Efficient synthesis of double dye-labeled oligodeoxyribonucleotide probes and their application in a real time PCR assay.

Authors:  B Mullah; K Livak; A Andrus; P Kenney
Journal:  Nucleic Acids Res       Date:  1998-02-15       Impact factor: 16.971

4.  Rainbow Universal CPG: a versatile solid support for oligonucleotide synthesis.

Authors:  P S Nelson; S Muthini; M Vierra; L Acosta; T H Smith
Journal:  Biotechniques       Date:  1997-04       Impact factor: 1.993

5.  Rapid conditions for the cleavage of oligodeoxyribonucleotides from cis-diol-bearing universal polymer supports and their deprotection.

Authors:  P Kumar; K C Gupta
Journal:  Nucleic Acids Res       Date:  1999-05-15       Impact factor: 16.971

6.  Kinetic Analysis of Diamine-Catalyzed RNA Hydrolysis.

Authors:  Makoto Komiyama; Koichi Yoshinari
Journal:  J Org Chem       Date:  1997-04-04       Impact factor: 4.354

7.  Oxalyl-CPG: a labile support for synthesis of sensitive oligonucleotide derivatives.

Authors:  R H Alul; C N Singman; G R Zhang; R L Letsinger
Journal:  Nucleic Acids Res       Date:  1991-04-11       Impact factor: 16.971

8.  Hairpin ribozyme cleavage catalyzed by aminoglycoside antibiotics and the polyamine spermine in the absence of metal ions.

Authors:  D J Earnshaw; M J Gait
Journal:  Nucleic Acids Res       Date:  1998-12-15       Impact factor: 16.971

9.  On the rapid deprotection of synthetic oligonucleotides and analogs.

Authors:  N N Polushin; A M Morocho; B C Chen; J S Cohen
Journal:  Nucleic Acids Res       Date:  1994-02-25       Impact factor: 16.971

10.  Deprotection of methylphosphonate oligonucleotides using a novel one-pot procedure.

Authors:  R I Hogrefe; M M Vaghefi; M A Reynolds; K M Young; L J Arnold
Journal:  Nucleic Acids Res       Date:  1993-05-11       Impact factor: 16.971
  10 in total
  1 in total

1.  Non-enzymatic template-directed recombination of RNAs.

Authors:  Sergey Y Nechaev; Alexei V Lutay; Valentin V Vlassov; Marina A Zenkova
Journal:  Int J Mol Sci       Date:  2009-04-21       Impact factor: 6.208
  1 in total

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