| Literature DB >> 12461562 |
James A Brannigan1, Anthony J Wilkinson.
Abstract
It is 20 years since site-directed mutagenesis was first used to modify the active site of an enzyme of known structure and mechanism. Since then, this method has contributed far-reaching insights into catalysis, specificity, stability and folding of proteins. Engineered proteins are now being used in industry and for the improved treatment of human disease.Entities:
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Year: 2002 PMID： 12461562 DOI： 10.1038/nrm975
Source DB: PubMed Journal: Nat Rev Mol Cell Biol ISSN： 1471-0072 Impact factor: 94.444