Literature DB >> 12450871

Competitive PCR for quantitation of a Cytophaga-Flexibacter-Bacteroides phylum bacterium associated with the Tuber borchii Vittad. mycelium.

Elena Barbieri1, Giulia Riccioni, Anna Pisano, Davide Sisti, Sabrina Zeppa, Deborah Agostini, Vilberto Stocchi.   

Abstract

An uncultured bacterium associated with the ectomycorrhizal fungus Tuber borchii Vittad. was identified as a novel member of the Cytophaga-Flexibacter-Bacteroides group. Utilizing a quantitative PCR targeting the 16S rRNA gene, we relatively quantified this bacterium in the host. The estimated number of bacteria was found to be approximately 10(6) cells per 30-day-old T. borchii mycelium culture. This represents the first molecular attempt to enumerate an uncultured bacterium associated with a mycorrhizal fungus.

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Year:  2002        PMID： 12450871      PMCID： PMC134394          DOI： 10.1128/AEM.68.12.6421-6424.2002

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

1.  rrndb: the Ribosomal RNA Operon Copy Number Database.

Authors:  J A Klappenbach; P R Saxman; J R Cole; T M Schmidt
Journal:  Nucleic Acids Res       Date:  2001-01-01       Impact factor: 16.971

2.  Basic principles of quantitative PCR.

Authors:  L Raeymaekers
Journal:  Mol Biotechnol       Date:  2000-06       Impact factor: 2.695

3.  Quantitative selective PCR of 16S ribosomal DNA correlates well with selective agar plating in describing population dynamics of indigenous Pseudomonas spp. in soil hot spots.

Authors:  K Johnsen; O Enger; C S Jacobsen; L Thirup; V Torsvik
Journal:  Appl Environ Microbiol       Date:  1999-04       Impact factor: 4.792

4.  Isolation and characterization of differentially expressed genes in the mycelium and fruit body of Tuber borchii.

Authors:  Isabelle Lacourt; Sébastien Duplessis; Simona Abbà; Paola Bonfante; Francis Martin
Journal:  Appl Environ Microbiol       Date:  2002-09       Impact factor: 4.792

5.  Identification of differentially expressed cDNA clones in Tilia platyphyllos-Tuber borchii ectomycorrhizae using a differential screening approach.

Authors:  E Polidori; D Agostini; S Zeppa; L Potenza; F Palma; D Sisti; V Stocchi
Journal:  Mol Genet Genomics       Date:  2001-11-16       Impact factor: 3.291

6.  Survival and activity of Pseudomonas sp. strain B13(FR1) in a marine microcosm determined by quantitative PCR and an rRNA-targeting probe and its effect on the indigenous bacterioplankton.

Authors:  T D Leser; M Boye; N B Hendriksen
Journal:  Appl Environ Microbiol       Date:  1995-04       Impact factor: 4.792

7.  Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.

Authors:  J Brosius; M L Palmer; P J Kennedy; H F Noller
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

8.  Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples.

Authors:  V Zachar; R A Thomas; A S Goustin
Journal:  Nucleic Acids Res       Date:  1993-04-25       Impact factor: 16.971

9.  Effect of genome size and rrn gene copy number on PCR amplification of 16S rRNA genes from a mixture of bacterial species.

Authors:  V Farrelly; F A Rainey; E Stackebrandt
Journal:  Appl Environ Microbiol       Date:  1995-07       Impact factor: 4.792

10.  Natural relationship between bacteroides and flavobacteria.

Authors:  W G Weisburg; Y Oyaizu; H Oyaizu; C R Woese
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490
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  1 in total

1.  Bacteria of the genus Rhodopseudomonas (Bradyrhizobiaceae): obligate symbionts in mycelial cultures of the black truffles Tuber melanosporum and Tuber brumale.

Authors:  Christine Le Roux; Estelle Tournier; Adrien Lies; Hervé Sanguin; Gérard Chevalier; Robin Duponnois; Daniel Mousain; Yves Prin
Journal:  Springerplus       Date:  2016-07-15
  1 in total

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